Clonotypic heterogeneity of lewis rat T cells specific for the encephalitogenic 68-86 region of myelin basic protein
dc.contributor.author | Mannie, M. D. | en_US |
dc.contributor.author | Paterson, P. Y. | en_US |
dc.contributor.author | U'Prichard, D. C. | en_US |
dc.contributor.author | Thomas, David W. | en_US |
dc.date.accessioned | 2006-04-07T20:43:03Z | |
dc.date.available | 2006-04-07T20:43:03Z | |
dc.date.issued | 1989-09 | en_US |
dc.identifier.citation | Mannie, M. D., Paterson, P. Y., U'Prichard, D. C., Thomas, D. W. (1989/09)."Clonotypic heterogeneity of lewis rat T cells specific for the encephalitogenic 68-86 region of myelin basic protein." Cellular Immunology 122(2): 534-547. <http://hdl.handle.net/2027.42/27792> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WCF-4F7XG2H-42/2/60139e2b10fc0071d3a3bb3a5b8fa4a6 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27792 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2475260&dopt=citation | en_US |
dc.description.abstract | Experimental autoimmune encephalomyelitis was induced in a Lewis rat by sensitization with synthetic peptide GP68-86, representing the 68-86 sequence of guinea pig myelin basic protein (GPMBP). To delineate T cell determinants of GP68-86, lymph node cells from this rat were activated in culture with GP68-86 and were fused with cells of the mouse thymoma BW5147. The resultant hybrids were cloned by limiting dilution and screened for GP68-86-evoked secretion of IL 2 in the presence of rat splenocytes. Twelve T cell hybrids derived in this manner were tested for reactivity to different heterologous species of MBP as well as to substituted or truncated analogs of GP68-86. The hybrids generally exhibited potent reactivity to GPMBP but differed markedly in their reactivity to autologous rat MBP (RMBP). A few exceptional hybrids exhibited crossreactivity with peptides in which native serine75 or serine80 residues of GPMBP were substituted with either alanine75 (A75) or proline80 (P80) residues. These cross-reactive hybrids also possessed high levels of anti-RMBP reactivity. The remaining hybrids were unresponsive to the A75 and P80 substituted peptides and, with one exception, had relatively low levels of anti-RMBP reactivity. Unique reactivity patterns were also revealed by hybrid responses to peptides having modified C-terminal 84-86 residues. In summary, the contrasting fine specificities of different hybrids indicated that several distinct clones of T cells mediate the immune response of Lewis rats against the 68-86 region of GPMBP. Furthermore, heterogeneity in the hybrid response to "self" RMBP may reflect substantial differences in encephalitogenic potency of the T cell clones from which these hybrids were derived. | en_US |
dc.format.extent | 1039865 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Clonotypic heterogeneity of lewis rat T cells specific for the encephalitogenic 68-86 region of myelin basic protein | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Microbiology-Immunology, Medical and Dental Schools, Northwestern University, Chicago, Illinois 60611, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Neurobiology and Physiology, College of Arts and Sciences, Northwestern University, Evanston, Illinois 60201, U.S.A. | en_US |
dc.identifier.pmid | 2475260 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27792/1/0000190.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0008-8749(89)90099-3 | en_US |
dc.identifier.source | Cellular Immunology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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