Cyclic AMP-dependent protein kinase decreases GABAA receptor current in mouse spinal neurons
dc.contributor.author | Porter, Nada M. | en_US |
dc.contributor.author | Twyman, Roy E. | en_US |
dc.contributor.author | Uhler, Michael D. | en_US |
dc.contributor.author | Macdonald, Robert L. | en_US |
dc.date.accessioned | 2006-04-10T13:33:06Z | |
dc.date.available | 2006-04-10T13:33:06Z | |
dc.date.issued | 1990-12 | en_US |
dc.identifier.citation | Porter, Nada M., Twyman, Roy E., Uhler, Michael D., Macdonald, Robert L. (1990/12)."Cyclic AMP-dependent protein kinase decreases GABAA receptor current in mouse spinal neurons." Neuron 5(6): 789-796. <http://hdl.handle.net/2027.42/28296> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WSS-4D5MN2N-H/2/cc20a5e0e8de8b16cf81687865fe75e5 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28296 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2176510&dopt=citation | en_US |
dc.description.abstract | GABA, the major inhibitory neurotransmitter in the mammalian brain, binds to GABAA receptors, which form chloride ion channels. The predicted structure of the GABAA receptor places a consensus phosphorylation site for cAMP-dependent protein kinase (PKA) on an intracellular domain of the channel. Phosphorylation by various protein kinases has been shown to alter the activity of certain ligand- and voltage-gated ion channels. We have examined the role of phosphorylation by the catalytic subunit of PKA in the regulation of GABAA receptor channel function using whole-cell and excised outside-out patch-clamp techniques. Inclusion of the catalytic subunit of PKA in the recording pipettes significantly reduced GABA-evoked whole-cell and single-channel chloride currents. Both heat inactivation of PKA and addition of the specific protein kinase inhibitor peptide prevented the reduction of GABA-evoked currents by PKA. Neither mean channel open time nor channel conductance was affected by PKA. The reduction in GABA receptor current by PKA was primarily due to a reduction in channel opening frequency. | en_US |
dc.format.extent | 881640 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Cyclic AMP-dependent protein kinase decreases GABAA receptor current in mouse spinal neurons | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Neurology University of Michigan Medical Center, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationum | Departments of Neurology University of Michigan Medical Center, Ann Arbor, Michigan 48104, USA | en_US |
dc.contributor.affiliationum | Mental Health Research Institute University of Michigan Medical Center, Ann Arbor, Michigan 48104, USA; Department of Biological Chemistry University of Michigan Medical Center, Ann Arbor, Michigan 48104, USA. | en_US |
dc.contributor.affiliationum | Department of Physiology University of Michigan Medical Center, Ann Arbor, Michigan 48104, USA; Departments of Neurology University of Michigan Medical Center, Ann Arbor, Michigan 48104, USA. | en_US |
dc.identifier.pmid | 2176510 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28296/1/0000050.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0896-6273(90)90338-G | en_US |
dc.identifier.source | Neuron | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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