Dependence of an alkyl glycol-ether monooxygenase activity upon tetrahydropterins
dc.contributor.author | Kaufman, Seymour | en_US |
dc.contributor.author | Pollock, Robert J. | en_US |
dc.contributor.author | Summer, George K. | en_US |
dc.contributor.author | Das, Arun K. | en_US |
dc.contributor.author | Hajra, Amiya K. | en_US |
dc.date.accessioned | 2006-04-10T13:38:27Z | |
dc.date.available | 2006-04-10T13:38:27Z | |
dc.date.issued | 1990-08-01 | en_US |
dc.identifier.citation | Kaufman, Seymour, Pollock, Robert J., Summer, George K., Das, Arun K., Hajra, Amiya K. (1990/08/01)."Dependence of an alkyl glycol-ether monooxygenase activity upon tetrahydropterins." Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 1040(1): 19-27. <http://hdl.handle.net/2027.42/28430> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T21-47T1N1F-4/2/612efd2cdf469bcade8f99ec56eecaba | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28430 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2378898&dopt=citation | en_US |
dc.description.abstract | Glyceryl-ether monooxygenase (1-alkyl-sn-glycerol,tetrahydropteridine:oxygen oxidoreductase, EC 1.14.16.5) catalyzes the oxidative cleavage of 1-O-alkyl glycerol or glycol derivatives to a long-chain aldehyde and the glycerol or glycol derivative. The specificity for tetrahydropterins of a similar, perhaps identical, enzyme that cleaves O-hexadecyl ethylene glycol in rat liver microsomes was examined with the use of an assay based on [1-3H]ethylene glycol formation from 2-hexadecyloxy[1-3H]ethan-1-ol. Several tetrahydropterin derivatives are effective electron donors for this reaction, and 2,4,5-triamino-6-hydroxypyrimidine is somewhat effective, but NADH, NADPH, ascorbate, reduced dichlorophenolindophenol and glutathione are inactive. Tetrahydropterin derivatives differ from each other in apparent Km and apparent Vmax. The order of increasing apparent Km values is tetrahydropterin [approximate] 6-methyltetrahydropterine [approximate] tetrahydrobiopterin Vmax values is tetrahydrofolate [approximate] tetrahydropterin P-450-dependent hydroxylases, this alkyl glycol-ether monooxogenase is not inhibited by carbon monoxide. 1-O-hexadecyl-rac-glycerol (chimyl alcohol) competitively inhibits the oxidation of the glycol ether indicating that the same enzyme probably catalyzes the oxidation of both O-alkyl glycol and 1-O-alkyl glycerol. | en_US |
dc.format.extent | 821889 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Dependence of an alkyl glycol-ether monooxygenase activity upon tetrahydropterins | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory, Mental Health Research Institute and Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory, Mental Health Research Institute and Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationother | Laboratory of Neurochemistry, NIMH, NIH, Bethesda, MD, U.S.A. | en_US |
dc.contributor.affiliationother | Laboratory of Neurochemistry, NIMH, NIH, Bethesda, MD, U.S.A. | en_US |
dc.contributor.affiliationother | Laboratory of Neurochemistry, NIMH, NIH, Bethesda, MD, U.S.A. | en_US |
dc.identifier.pmid | 2378898 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28430/1/0000216.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0167-4838(90)90141-2 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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