Rabbit nasal cytochrome P-450 NMa has high activity as a nicotine oxidase

Williams, David E.; Ding, Xinxin; Coon, Minor J.

1990-01-30

Citation: Williams, David E., Ding, Xinxin, Coon, Minor J. (1990/01/30)."Rabbit nasal cytochrome P-450 NMa has high activity as a nicotine oxidase." Biochemical and Biophysical Research Communications 166(2): 945-952. <http://hdl.handle.net/2027.42/28747>

Abstract: Rabbit nasal olfactory and respiratory microsomes demonstrate high activity toward [3H]-(S)-nicotine, with specific activities of 22.2 and 6.5 nmol/min/mg protein, respectively. The major metabolite produced is (S)-nicotine [Delta]I',5'-iminium ion, with lesser amounts of nornicotine and the N'-oxide. Reconstitution of the rabbit nasal microsomal system with cytochromes P-450 NMa and NMb indicated that only P-450 NMa has significant activity toward nicotine, and the metabolite profile and turnover are similar to that observed with nasal microsomes. The low Km (35 [mu]M) and high Vmax (28 min-1) suggest that a significant portion of inhaled nicotine is metabolized by nasal tissues in the rabbit.

DOIs: http://dx.doi.org/10.1016/0006-291X(90)90902-Y

PMID: 2302249

URI: http://www.sciencedirect.com/science/article/B6WBK-4DMX9RM-155/2/738dbf11808dfc7ab5523d063669bd84
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2302249&dopt=citation

Handle: http://hdl.handle.net/2027.42/28747

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