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Rabbit nasal cytochrome P-450 NMa has high activity as a nicotine oxidase

dc.contributor.authorWilliams, David E.en_US
dc.contributor.authorDing, Xinxinen_US
dc.contributor.authorCoon, Minor J.en_US
dc.date.accessioned2006-04-10T13:51:03Z
dc.date.available2006-04-10T13:51:03Z
dc.date.issued1990-01-30en_US
dc.identifier.citationWilliams, David E., Ding, Xinxin, Coon, Minor J. (1990/01/30)."Rabbit nasal cytochrome P-450 NMa has high activity as a nicotine oxidase." Biochemical and Biophysical Research Communications 166(2): 945-952. <http://hdl.handle.net/2027.42/28747>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WBK-4DMX9RM-155/2/738dbf11808dfc7ab5523d063669bd84en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/28747
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2302249&dopt=citationen_US
dc.description.abstractRabbit nasal olfactory and respiratory microsomes demonstrate high activity toward [3H]-(S)-nicotine, with specific activities of 22.2 and 6.5 nmol/min/mg protein, respectively. The major metabolite produced is (S)-nicotine [Delta]I',5'-iminium ion, with lesser amounts of nornicotine and the N'-oxide. Reconstitution of the rabbit nasal microsomal system with cytochromes P-450 NMa and NMb indicated that only P-450 NMa has significant activity toward nicotine, and the metabolite profile and turnover are similar to that observed with nasal microsomes. The low Km (35 [mu]M) and high Vmax (28 min-1) suggest that a significant portion of inhaled nicotine is metabolized by nasal tissues in the rabbit.en_US
dc.format.extent575172 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleRabbit nasal cytochrome P-450 NMa has high activity as a nicotine oxidaseen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, Medical School, The University of Michigan, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, Medical School, The University of Michigan, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationotherDepartment of Food Science & Technology, Oregon State University, Corvallis, OR 97331, USA; Toxicology Program, Oregon State University, Corvallis, OR 97331, USA.en_US
dc.identifier.pmid2302249en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/28747/1/0000577.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0006-291X(90)90902-Yen_US
dc.identifier.sourceBiochemical and Biophysical Research Communicationsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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