Stereoselective high-performance liquid chromatographic assay for pirmenol enantiomers in dog plasma
dc.contributor.author | Janiczek, Nancy | en_US |
dc.contributor.author | Bockbrader, Howard N. | en_US |
dc.contributor.author | Chang, Tsun | en_US |
dc.contributor.author | Amidon, Gordon L. | en_US |
dc.contributor.author | Smith, David E. | en_US |
dc.date.accessioned | 2006-04-10T14:28:40Z | |
dc.date.available | 2006-04-10T14:28:40Z | |
dc.date.issued | 1991-12-15 | en_US |
dc.identifier.citation | Janiczek, Nancy, Bockbrader, Howard N., Chang, Tsun, Amidon, Gordon L., Smith, David E. (1991/12/15)."Stereoselective high-performance liquid chromatographic assay for pirmenol enantiomers in dog plasma." Journal of Chromatography: Biomedical Applications 571(1-2): 179-187. <http://hdl.handle.net/2027.42/28977> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TG9-457CHXR-K/2/adf5102140bd66ab106368d9283c69b5 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28977 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1810946&dopt=citation | en_US |
dc.description.abstract | Pirmenol enantiomers in dog plasma were quantified using a stereospecific high-performance liquid chromatographic method with ultraviolet detection at 262 nm. Racemic pirmenol and internal standard, (+)-propranolol, were isolated from dog plasma by a three-step extraction procedure using toluene, 0.1 M hydrochloric acid and hexane, respectively. A chiral analytical column (Chiralcel OJ) was used with a mobile phase consisting of hexane--isopropanol--diethylamine (98.9:1.0:0.1). Linear calibration curves were obtained in the concentration range 0.0200-5.00 [mu]g/ml for each enantiomer. Precision of the method, expressed as coefficient of variation for nine quality control samples, was 7.1% for (+)-pirmenol and 6.4% for (-)-pirmenol. Bias was +/-2.2% for (+)-pirmenol and +/-1.5% for (-)-pirmenol in quality control samples. | en_US |
dc.format.extent | 451404 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Stereoselective high-performance liquid chromatographic assay for pirmenol enantiomers in dog plasma | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Pharmacokinetics/Drug Metabolism Department, Parke-Davis Pharmaceutical Research Division, Warner Lambert Company, Ann Arbor, MI 48105 USA; College of Pharmacy, University of Michigan, 428 Church Street, Ann Arbor, MI 48109 USA. | en_US |
dc.contributor.affiliationum | College of Pharmacy, The University of Michigan, 428 Church Street, Ann Arbor, MI 48109 USA | en_US |
dc.contributor.affiliationum | College of Pharmacy, The University of Michigan, 428 Church Street, Ann Arbor, MI 48109 USA | en_US |
dc.contributor.affiliationum | College of Pharmacy, The University of Michigan, 428 Church Street, Ann Arbor, MI 48109 USA | en_US |
dc.contributor.affiliationum | College of Pharmacy, The University of Michigan, 428 Church Street, Ann Arbor, MI 48109 USA | en_US |
dc.identifier.pmid | 1810946 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28977/1/0000004.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0378-4347(91)80444-H | en_US |
dc.identifier.source | Journal of Chromatography: Biomedical Applications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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