Molecular cloning of the human histamine H2 receptor
dc.contributor.author | Gantz, Ira | en_US |
dc.contributor.author | Munzert, Gerd | en_US |
dc.contributor.author | Tashiro, Takao | en_US |
dc.contributor.author | Schaffer, Matthias | en_US |
dc.contributor.author | Wang, Lidong | en_US |
dc.contributor.author | DelValle, John | en_US |
dc.contributor.author | Yamada, Tadataka | en_US |
dc.date.accessioned | 2006-04-10T14:37:03Z | |
dc.date.available | 2006-04-10T14:37:03Z | |
dc.date.issued | 1991-08-15 | en_US |
dc.identifier.citation | Gantz, Ira, Munzert, Gerd, Tashiro, Takao, Schaffer, Matthias, Wang, Lidong, DelValle, John, Yamada, Tadataka (1991/08/15)."Molecular cloning of the human histamine H2 receptor." Biochemical and Biophysical Research Communications 178(3): 1386-1392. <http://hdl.handle.net/2027.42/29176> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4DNJ4Y4-2J7/2/0f64038a708b4840c58d52756092c166 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29176 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1714721&dopt=citation | en_US |
dc.description.abstract | We utilized the technique of polymerase chain reaction with oligonucleotide primers based upon the nucleotide sequence of the canine H2 histamine receptor gene which we recently isolated to clone its human homologue. Transfection of a construct of this gene in Colo-320 DM cells led to the expression of a receptor that bound to [methyl-3H] tiotidine and was linked to 3',5' cyclic adenosine monophosphate (cAMP) generation in response to histamine. Both cAMP generation and [methyl-3H] tiotidine binding were inhibited with the H2 histamine receptor selective antagonist cimetidine but not diphenhydramine or thioperamide which are, respectively, H1 and H3 histamine receptor antagonists. These data confirm that we have successfully cloned a novel gene encoding the human H2 histamine receptor. | en_US |
dc.format.extent | 739673 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Molecular cloning of the human histamine H2 receptor | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Physiology, University of Michigan Medical Center, Ann Arbor, MI, USA | en_US |
dc.identifier.pmid | 1714721 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29176/1/0000223.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-291X(91)91047-G | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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