Inhibitory effects of somatostatin on rat hepatocyte proliferation are mediated by cyclic AMP,
dc.contributor.author | Kokudo, Norihiro | en_US |
dc.contributor.author | Kothary, Piyush C. | en_US |
dc.contributor.author | Eckhauser, Frederic E. | en_US |
dc.contributor.author | Raper, Steven E. | en_US |
dc.date.accessioned | 2006-04-10T14:37:25Z | |
dc.date.available | 2006-04-10T14:37:25Z | |
dc.date.issued | 1991-08 | en_US |
dc.identifier.citation | Kokudo, Norihiro, Kothary, Piyush C., Eckhauser, Frederic E., Raper, Steven E. (1991/08)."Inhibitory effects of somatostatin on rat hepatocyte proliferation are mediated by cyclic AMP,." Journal of Surgical Research 51(2): 113-118. <http://hdl.handle.net/2027.42/29185> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WM6-4BNG379-TH/2/77e9dde883f1a6bef7a44bb34768fb4a | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29185 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1713980&dopt=citation | en_US |
dc.description.abstract | Somatostatin (SS-14) is known as an antigrowth factor for a variety of cell types, including gastrointestinal mucosa, exocrine pancreas, lymphocytes, and some tumors. We have recently identified and biochemically characterized SS-14-binding protein on rat liver plasma membranes (S. E. Raper, P. C. Kothary, and J. DelValle, Gastroenterology 96: A408, 1989; P. C. Kothary et al., Digestion 46(Suppl 1): 58, 1990). We hypothesized that SS-14 may affect liver growth as well and investigated cellular mechanisms of this phenomenon focusing on the second messenger cAMP. Freshly isolated rat hepatocytes were plated on tissue culture dishes coated with Matrigel (laminin, heparan sulfate, and type IV collagen). The medium was not supplemented with serum or hormones. Either dibutyryl-cAMP (1 mM) or isobutylmethylxanthine (IBMX, 0.1 mM) was added in the presence or absence of SS-14 (10 nM). DNA synthesis was estimated by the rate of [3H]thymidine incorporation into DNA and by the labeling index (an autoradiographic measurement of the number of labeled nuclei). SS-14 significantly inhibited both [3H]thymidine incorporation and labeling index of rat hepatocytes stimulated by dibutyryl-cAMP or IBMX. SS-14 also inhibited intracellular cAMP accumulation stimulated by IBMX. We conclude that SS-14 exerts at least part of its antiproliferative effects via the adenylate cyclase system. Further study using other signal transduction systems may yield more information about mechanisms of hepatocyte growth. | en_US |
dc.format.extent | 706924 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Inhibitory effects of somatostatin on rat hepatocyte proliferation are mediated by cyclic AMP, | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Surgery and Anesthesiology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 1713980 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29185/1/0000238.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0022-4804(91)90079-2 | en_US |
dc.identifier.source | Journal of Surgical Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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