Protease nexin-1 activity in cultured Schwann cells
dc.contributor.author | Mulligan, L. P. | en_US |
dc.contributor.author | Rosenblatt, D. E. | en_US |
dc.contributor.author | Toms, R. | en_US |
dc.contributor.author | Johnson, D. | en_US |
dc.date.accessioned | 2006-04-10T14:39:15Z | |
dc.date.available | 2006-04-10T14:39:15Z | |
dc.date.issued | 1991-07-08 | en_US |
dc.identifier.citation | Mulligan, L. P., Rosenblatt, D. E., Toms, R., Johnson, D. (1991/07/08)."Protease nexin-1 activity in cultured Schwann cells." Neuroscience Letters 128(1): 42-46. <http://hdl.handle.net/2027.42/29228> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T0G-485TW6B-3PC/2/d1c4863cdde699d3e76b65eb90d9ca20 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29228 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1717897&dopt=citation | en_US |
dc.description.abstract | We report that protease nexin-1 (PN-1), a serine protease inhibitor known to have neurite-promoting effects, is made by Schwann cells in tissue culture. Three modalities have been used to demonstrate the presence of PN-1 in Schwann cell cultures. Immunostaining of the cultures with anti-PN-1 antibody gives positive staining over cells and matrix. Western blots of Schwann cell conditioned medium (CM) using anti-PN-1 antibody show a band that co-migrates with the PN-1 standard at 45 kDa. Biochemical assay for protease inhibitory activity shows that CM inhibits thrombin activity in a calorimetric assay. The CM-mediated inhibition of thrombin is reversed if the CM is pre-incubated with anti-PN-1 antibody. | en_US |
dc.format.extent | 533326 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Protease nexin-1 activity in cultured Schwann cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationother | Center for Neurologic Disease, Brigham and Women's Hospital, Boston, MA 02215, U.S.A. | en_US |
dc.contributor.affiliationother | Center for Neurologic Disease, Brigham and Women's Hospital, Boston, MA 02215, U.S.A. | en_US |
dc.contributor.affiliationother | Center for Neurologic Disease, Brigham and Women's Hospital, Boston, MA 02215, U.S.A. | en_US |
dc.identifier.pmid | 1717897 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29228/1/0000283.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0304-3940(91)90756-J | en_US |
dc.identifier.source | Neuroscience Letters | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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