The major component of a large, intracellular proteinase accumulated by inhibitors is a complex of [alpha]2-macroglobulin and thrombin
dc.contributor.author | Tsuji, A. | en_US |
dc.contributor.author | Arai, T. | en_US |
dc.contributor.author | Furcinitti, P. S. | en_US |
dc.contributor.author | Langmore, John P. | en_US |
dc.contributor.author | Kurachi, Kotoku | en_US |
dc.date.accessioned | 2006-04-10T14:43:05Z | |
dc.date.available | 2006-04-10T14:43:05Z | |
dc.date.issued | 1991-05-30 | en_US |
dc.identifier.citation | Tsuji, A., Arai, T., Furcinitti, P. S., Langmore, J. P., Kurachi, K. (1991/05/30)."The major component of a large, intracellular proteinase accumulated by inhibitors is a complex of [alpha]2-macroglobulin and thrombin." Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 1078(1): 85-93. <http://hdl.handle.net/2027.42/29323> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T21-486TCSX-2R/2/b8293048c80695023370b5b8328cab8b | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29323 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1710931&dopt=citation | en_US |
dc.description.abstract | A large, intracellular proteinase accumulated by inhibitors (PABI) was found in cultured mammalian cells as a large, multicatalytic proteinase with a greatly elevated concentration in the presence of small peptide proteinase inhibitors (Tsuji and Kurachi (1989) J. Biol. Chem. 264, 16093). Electron microscopic analysis showed that the tertiary structure of PABI highly resembled that of [alpha]2-macroglobulin complexed with a proteinase(s). Isolation of the anti-PABI cross-reacting material from calf serum added to the culture media of baby hamster kidney cells further supported that the primary component of PABI was [alpha]2-macroglobulin. Immunoblot analyses and the substrate specificity of PABI indicated that the major proteinase component contained in PABI was thrombin. When [alpha]2-macroglobulin was added to the PABI-depleted serum, a significant accumulation or a degradation of the intracellular [alpha]2-macroglobulin was observed in the presence or absence of leupeptin, respectively. Similarly, when thrombin was added to the PABI-depleted fetal calf serum supplemented with fresh [alpha]2-macroglobulin, a significant amount of intracellular thrombin was found only in the presence of leupeptin. These results indicate that the major component of the intracellular PABI molecules is a complex of [alpha]2-macroglobulin with thrombin which is internalized from the culture media. Intracellular accumulation of PABI, therefore, is a phenomenon primarily relevant to the culture cells. Whether or not PABI is also generated in certain physiological or pathological conditions requires further study. | en_US |
dc.format.extent | 604532 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | The major component of a large, intracellular proteinase accumulated by inhibitors is a complex of [alpha]2-macroglobulin and thrombin | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics and Biophysics Research Division, University of Michigan, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Human Genetics and Biophysics Research Division, University of Michigan, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Human Genetics and Biophysics Research Division, University of Michigan, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Human Genetics and Biophysics Research Division, University of Michigan, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Human Genetics and Biophysics Research Division, University of Michigan, Ann Arbor, MI, U.S.A. | en_US |
dc.identifier.pmid | 1710931 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29323/1/0000388.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0167-4838(91)90096-I | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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