Structure of the human cytochrome c oxidase subunit Vb gene and chromosomal mapping of the coding gene and of seven pseudogenes
dc.contributor.author | Lomax, Margaret I. | en_US |
dc.contributor.author | Hsieh, Chih-Lin | en_US |
dc.contributor.author | Darras, Basil T. | en_US |
dc.contributor.author | Francke, Uta | en_US |
dc.date.accessioned | 2006-04-10T14:43:41Z | |
dc.date.available | 2006-04-10T14:43:41Z | |
dc.date.issued | 1991-05 | en_US |
dc.identifier.citation | Lomax, Margaret I., Hsieh, Chih-Lin, Darras, Basil T., Francke, Uta (1991/05)."Structure of the human cytochrome c oxidase subunit Vb gene and chromosomal mapping of the coding gene and of seven pseudogenes." Genomics 10(1): 1-9. <http://hdl.handle.net/2027.42/29338> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WG1-4DP5JB1-1/2/fed94c7b0ec1454aa61136c9ec975b5f | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29338 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1646156&dopt=citation | en_US |
dc.description.abstract | Subunit Vb of mammalian cytochrome c oxidase (COX; EC 1.9.3.1) is encoded by a nuclear gene and assembled with the other 12 COX subunits encoded in both mitochondrial and nuclear DNA. We have cloned the gene for human COX subunit Vb (COX5B) and determined the exon-intron structure by both hybridization analysis and DNA sequencing. The gene contains five exons and four introns; the four coding exons span a region of approximately 2.4 kb. The 5' end of the COX5B gene is GC-rich and contains many HpaII sites. Genomic Southern blot analysis of human DNA probed with the human COX Vb cDNA identified eight restriction fragments containing COX Vb-related sequences that were mapped to different chromosomes with panels of human x Chinese hamster somatic cell hybrids. Because only one of these fragments hybridized with a 210-bp probe from intron 4, we conclude that there is a single expressed gene for COX subunit Vb in the human genome. We have mapped this gene to chromosome 2, region cen-q13. | en_US |
dc.format.extent | 2468651 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Structure of the human cytochrome c oxidase subunit Vb gene and chromosomal mapping of the coding gene and of seven pseudogenes | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationother | Howard Hughes Medical Institute, Stanford University Medical Center, Stanford, California 94305, USA; Department of Genetics, Stanford University Medical Center, Stanford, California 94305, USA. | en_US |
dc.contributor.affiliationother | Department of Pediatrics, New England Medical Center, Boston, Massachusetts 02111, USA | en_US |
dc.contributor.affiliationother | Howard Hughes Medical Institute, Stanford University Medical Center, Stanford, California 94305, USA; Department of Genetics, Stanford University Medical Center, Stanford, California 94305, USA. | en_US |
dc.identifier.pmid | 1646156 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29338/1/0000405.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0888-7543(91)90476-U | en_US |
dc.identifier.source | Genomics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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