Show simple item record

cDNA sequence and genomic structure of EVI2B, a gene lying within an intron of the neurofibromatosis type 1 gene

dc.contributor.authorCawthon, Richard M.en_US
dc.contributor.authorAndersen, Lone B.en_US
dc.contributor.authorBuchberg, Arthur M.en_US
dc.contributor.authorXu, Gangfengen_US
dc.contributor.authorO'Connell, Peteren_US
dc.contributor.authorViskochil, David H.en_US
dc.contributor.authorWeiss, Robert B.en_US
dc.contributor.authorWallace, Margaret R.en_US
dc.contributor.authorMarchuk, Douglas A.en_US
dc.contributor.authorCulver, Melanieen_US
dc.date.accessioned2006-04-10T14:47:42Z
dc.date.available2006-04-10T14:47:42Z
dc.date.issued1991-03en_US
dc.identifier.citationCawthon, Richard M., Andersen, Lone B., Buchberg, Arthur M., Xu, Gangfeng, O'Connell, Peter, Viskochil, David, Weiss, Robert B., Wallace, Margaret R., Marchuk, Douglas A., Culver, Melanie (1991/03)."cDNA sequence and genomic structure of EVI2B, a gene lying within an intron of the neurofibromatosis type 1 gene." Genomics 9(3): 446-460. <http://hdl.handle.net/2027.42/29439>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WG1-4DXB903-4S/2/0c7b456ed86760a301a4e2d019252ea9en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/29439
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1903357&dopt=citationen_US
dc.description.abstractThe gene responsible for neurofibromatosis type 1 (NF1), one of the more common inherited human disorders, was identified recently, and segments of it were cloned. Two translocation breakpoints that interrupt the NF1 gene in NF1 patients flank a 60-kb segment of DNA that contains the EVI2A locus (previously reported as the EVI2 locus), the human homolog of a mouse gene, Evi-2A, implicated in retrovirus-induced murine myeloid tumors. EVI2A lies within an intron of the NF1 gene and is transcribed from telomere toward centromere, opposite to the direction of transcription of the NF1 gene. Here we describe a second locus, EVI2B, also located between the two NF1 translocation breakpoints. Full-length cDNAs from the EVI2B locus detect a 2.1-kb transcript in bone marrow, peripheral blood mononuclear cells, and fibroblasts. Sequencing studies predict an EVI2B protein of 448 amino acids that is proline-rich and contains an N-terminal signal peptide, an extracellular domain with four potential glycosylation sites, a single hydrophobic transmembrane domain, and a cytoplasmic hydrophilic domain. At the level of genomic DNA the EVI2B locus lies within the same intron of the NF1 gene as EVI2A and contains a 57-bp 5' exon that is noncoding, an 8-kb intron, and a 2078-bp 3' exon that includes the entire open reading frame. EVI2B is transcribed in the same direction as EVI2A; its 5' exon lies only 4 kb downstream from the 3' exon of the EVI2A locus. In the mouse the 5' exon of the homologous gene, Evi-2B, lies approximately 2.8 kb from the 3' end of Evi-2A, in the midst of a cluster of viral integration sites identified in retrovirus-induced myeloid tumors; thus, Evi-2B may function as an oncogene in these tumors.en_US
dc.format.extent2288048 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titlecDNA sequence and genomic structure of EVI2B, a gene lying within an intron of the neurofibromatosis type 1 geneen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelGeneticsen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumHoward Hughes Medical Institute, University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationotherHoward Hughes Medical Institute, University of Utah, Salt Lake City, Utah 84132, USAen_US
dc.contributor.affiliationotherHoward Hughes Medical Institute, University of Utah, Salt Lake City, Utah 84132, USAen_US
dc.contributor.affiliationotherHoward Hughes Medical Institute, University of Utah, Salt Lake City, Utah 84132, USAen_US
dc.contributor.affiliationotherHoward Hughes Medical Institute, University of Utah, Salt Lake City, Utah 84132, USAen_US
dc.contributor.affiliationotherHoward Hughes Medical Institute, University of Utah, Salt Lake City, Utah 84132, USAen_US
dc.contributor.affiliationotherHoward Hughes Medical Institute, University of Utah, Salt Lake City, Utah 84132, USAen_US
dc.identifier.pmid1903357en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/29439/1/0000521.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0888-7543(91)90410-Gen_US
dc.identifier.sourceGenomicsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.