Endothelin increases [Ca2+]i in rat pancreatic acinar cells by intracellular release but fails to increase amylase secretion
dc.contributor.author | Yule, David I. | en_US |
dc.contributor.author | Blevins, George T., Jr. | en_US |
dc.contributor.author | Wagner, Andreas C. C. | en_US |
dc.contributor.author | Williams, John A. | en_US |
dc.date.accessioned | 2006-04-10T15:07:23Z | |
dc.date.available | 2006-04-10T15:07:23Z | |
dc.date.issued | 1992-08-12 | en_US |
dc.identifier.citation | Yule, David I., Blevins, Jr., George T., Wagner, Andreas C., Williams, John A. (1992/08/12)."Endothelin increases [Ca2+]i in rat pancreatic acinar cells by intracellular release but fails to increase amylase secretion." Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1136(2): 175-180. <http://hdl.handle.net/2027.42/29904> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T20-4894KCM-C/2/589be323e3b548a84869154c164ebab5 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29904 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1380304&dopt=citation | en_US |
dc.description.abstract | In individual fura-2 loaded cells of rat pancreatic acini endothelin-1 (ET-1) (10-50 nM) induced sustained oscillations in [Ca2+]i. At higher concentrations a larger, but transient increase in [Ca2+]i was observed, which was largely unaffected by removal of extracellular Ca2+. ET-1 induced the release of Cai2+ from the same store as cholecystokinin (CCK), but with less potency. At concentrations of endothelin which transiently increased Ca2+, ET-1 increased the accumulation of inositol phosphates. Specific binding sites for 125I-endothelin were demonstrated on rat pancreatic acini. A single class of binding sites was identified with an apparent Kd 108 +/- 12 pM and Bmax of 171 +/- 17 fmol/mg for ET-1. The relative potency order for displacing [125I]ET was ET-1 > ET-2 > ET-3. In contrast to CCK and the non-phorbol ester tumour promoter Thapsigargin (TG) which induce both transient and sustained components of [Ca2+]i elevation, ET-1 failed to increase amylase release over the range 100 pM-1 [mu]M. | en_US |
dc.format.extent | 402714 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Endothelin increases [Ca2+]i in rat pancreatic acinar cells by intracellular release but fails to increase amylase secretion | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Physiology, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Physiology, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Physiology, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Physiology, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.identifier.pmid | 1380304 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29904/1/0000261.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0167-4889(92)90254-9 | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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