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Expressed sequence tags and chromosomal localization of cDNA clones from a subtracted retinal pigment epithelium library

dc.contributor.authorGieser, Linnen_US
dc.contributor.authorSwaroop, Ananden_US
dc.date.accessioned2006-04-10T15:10:13Z
dc.date.available2006-04-10T15:10:13Z
dc.date.issued1992-07en_US
dc.identifier.citationGieser, Linn, Swaroop, Anand (1992/07)."Expressed sequence tags and chromosomal localization of cDNA clones from a subtracted retinal pigment epithelium library." Genomics 13(3): 873-876. <http://hdl.handle.net/2027.42/29973>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WG1-4DNHS2F-RP/2/00b323379c27113a5434bed747afaa85en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/29973
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1639417&dopt=citationen_US
dc.description.abstractExpressed sequence tags (ESTs) provide useful molecular landmarks for physical mapping and identify the position of an expressed region in the genome. The use of subtracted cDNA libraries enriched for tissue-specific genes as a source of ESTs should reduce the repetitive isolation of constitutively expressed sequences. We report here the sequence tags from the 3'-end region of 58 new directionally cloned cDNAs from a substracted human retinal pigment epithelium (RPE) cell line library. Eight of the cDNAs have been assigned to human chromosomes using PCR-based EST assays. Chromosomal mapping of subtracted RPE cDNA clones may also help in identifying candidate genes for inherited eye diseases.en_US
dc.format.extent391477 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleExpressed sequence tags and chromosomal localization of cDNA clones from a subtracted retinal pigment epithelium libraryen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelGeneticsen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Ophthalmology, Kellogg Eye Center, University of Michigan, Ann Arbor, Michigan 48105, USA.en_US
dc.contributor.affiliationumDepartment of Ophthalmology, Kellogg Eye Center, University of Michigan, Ann Arbor, Michigan 48105, USA; Department of Human Genetics, Kellogg Eye Center, University of Michigan, Ann Arbor, Michigan 48105, USA.en_US
dc.identifier.pmid1639417en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/29973/1/0000336.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0888-7543(92)90173-Pen_US
dc.identifier.sourceGenomicsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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