Lactate Oxidation for the Detection of Mitochondrial Dysfunction in Human Skin Fibroblasts
dc.contributor.author | Ofenstein J. P. , | en_US |
dc.contributor.author | Kiechle F. L. , | en_US |
dc.contributor.author | Dandurand D. M. , | en_US |
dc.contributor.author | Belknap W. M. , | en_US |
dc.contributor.author | Moore K. H. , | en_US |
dc.contributor.author | Holmes R. D. , | en_US |
dc.date.accessioned | 2006-04-10T15:46:25Z | |
dc.date.available | 2006-04-10T15:46:25Z | |
dc.date.issued | 1993-05-01 | en_US |
dc.identifier.citation | Ofenstein J. P., , Kiechle F. L., , Dandurand D. M., , Belknap W. M., , Moore K. H., , Holmes R. D., (1993/05/01)."Lactate Oxidation for the Detection of Mitochondrial Dysfunction in Human Skin Fibroblasts." Analytical Biochemistry 210(2): 332-336. <http://hdl.handle.net/2027.42/30815> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6W9V-45R7DBH-81/2/b52d72070ae06fe09ac26a5a946631af | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/30815 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8512068&dopt=citation | en_US |
dc.description.abstract | To screen fibroblasts for defects in lactate/pyruvate oxidation, cells were grown to confluence in 25-cm2 flasks, rinsed, and incubated in glucose-free media containing 25 [mu]M L-lactate and 0.1 [mu]Ci [D,L-1-14C]lactate. Lactate oxidation was measured as the amount of lactate oxidized in nmol of 14CO2 generated /mg protein/min. Fibroblasts from patients with mitochondrial or peroxisomal disorders had decreased lactate oxidation compared to the control (CON): CON, 1.9 +/- 0.13 nmol/mg/min; neonatal adrenoleukodystrophy (NALD), 0.45 +/- 0.01 (P P P P < 0.001). This method is useful for screening fibroblasts for defects in lactate oxidation in patients with mitochondrial or peroxisomal disorders. Confirmation of the site of the defect may then be investigated with specific assays, e.g., PDH, in cellular homogenates: CON, 0.93 +/- 0.02 nmol/mg/min; NALD, 0.55 +/- 0.02; RCDP, 0.44 +/- 0.02; MIT, 0.53 +/- 0.03; PDH deficiency, 0.19 +/- 0.02. | en_US |
dc.format.extent | 446170 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Lactate Oxidation for the Detection of Mitochondrial Dysfunction in Human Skin Fibroblasts | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationother | William Beaumont Hospital, Royal Oak, MI 48073, USA | en_US |
dc.contributor.affiliationother | William Beaumont Hospital, Royal Oak, MI 48073, USA | en_US |
dc.contributor.affiliationother | William Beaumont Hospital, Royal Oak, MI 48073, USA | en_US |
dc.contributor.affiliationother | William Beaumont Hospital, Royal Oak, MI 48073, USA | en_US |
dc.contributor.affiliationother | Oakland University, Rochester, MI 48309, USA | en_US |
dc.identifier.pmid | 8512068 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/30815/1/0000475.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/abio.1993.1204 | en_US |
dc.identifier.source | Analytical Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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