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[alpha]-Thrombin within fibrin clots: Inactivation of thrombin by antithrombin-III

dc.contributor.authorNaski, Michael C.en_US
dc.contributor.authorShafer, Jules A.en_US
dc.date.accessioned2006-04-10T15:52:33Z
dc.date.available2006-04-10T15:52:33Z
dc.date.issued1993-03-01en_US
dc.identifier.citationNaski, Michael C., Shafer, Jules A. (1993/03/01)."[alpha]-Thrombin within fibrin clots: Inactivation of thrombin by antithrombin-III." Thrombosis Research 69(5): 453-465. <http://hdl.handle.net/2027.42/30953>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T1C-4C0DN7S-5/2/518cc422d6cd340ff093ee036310de7cen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/30953
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8497860&dopt=citationen_US
dc.description.abstractTo demonstrate that human [alpha]-thrombin is effectively inactivated by human antithrombin III (AT) during the production of a fibrin clot we measured the amount of a-thrombin activity which can be recovered from a clot generated from purified human proteins. We discovered that 0.05-0.07% of the original a-thrombin activity is recovered from a fibrin clot produced from a reaction mixture where the initial concentrations of AT and [alpha]-thrombin were chosen at a ratio (17.5) to allow complete conversion of fibrinogen to fibrin. These results indicated that [alpha]-thrombin is successfully inactivated by AT during the production of a fibrin clot. Further, when an amount of [alpha]-thrombin equal to that recovered from a fibrin clot is introduced into a solution of fibrinogen and AT identical to that utilized to produce the clot only 4% of the fibrinogen is converted to fibrin. These results suggest that i) when a fibrin clot is dissolved during fibrinolytic therapy little active [alpha]-thrombin should be released from the clot and ii) this amount of thrombin is insufficient to catalyze rethrombosis without proposing de novo production of thrombin. The action on factors XI, VIII, and V of the small amount of thrombin released upon thrombolysis, however, may provide the stimulus for de novo production of sufficient thrombin to catalyze rethrombosis.en_US
dc.format.extent1076882 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.title[alpha]-Thrombin within fibrin clots: Inactivation of thrombin by antithrombin-IIIen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelInternal Medicine and Specialtiesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, the University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationotherMerck Research Laboratories, WP16-101, Sumneytown Pike, West Point, Pennsylvania 19486, USAen_US
dc.identifier.pmid8497860en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/30953/1/0000625.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0049-3848(93)90234-Fen_US
dc.identifier.sourceThrombosis Researchen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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