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A Comparison of Mushroom Tyrosinase Dopaquinone and Dopachrome Assays Using Diode-Array Spectrophotometry: Dopachrome Formation vs Ascorbate-Linked Dopaquinone Reduction

dc.contributor.authorBehbahani I. ,en_US
dc.contributor.authorMiller S. A. ,en_US
dc.contributor.authorOkeeffe D. H. ,en_US
dc.date.accessioned2006-04-10T15:54:28Z
dc.date.available2006-04-10T15:54:28Z
dc.date.issued1993-02en_US
dc.identifier.citationBehbahani I., , Miller S. A., , Okeeffe D. H., (1993/02)."A Comparison of Mushroom Tyrosinase Dopaquinone and Dopachrome Assays Using Diode-Array Spectrophotometry: Dopachrome Formation vs Ascorbate-Linked Dopaquinone Reduction." Microchemical Journal 47(1-2): 251-260. <http://hdl.handle.net/2027.42/30995>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6W6H-45P0GVM-37/2/2ec8e6a83bf9b37f945b5db493be7eaaen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/30995
dc.description.abstractComparisons of two assay methods for the catecholase activity of mushroom tyrosinase (EC 1.14.18.1) are reported. Tyrosinase (or polyphenol oxidase) is a widely distributed copper-containing enzyme which possesses both monooxygenase (cresolase) and oxidase (catecholase) activities. In this report the substrate employed is L-3,4-dihydroxyphenyl-alanine and the dopachrome formation and ascorbate-linked dopaquinone reduction assay methods are compared using a photodiode array spectrophotometer. This instrument has an advantage over a conventional spectrometer for kinetic studies since it is able to carry out simultaneous multiwavelength kinetic measurements at a relatively fast rate. The use of this capability in performing the two assays is described.en_US
dc.format.extent411160 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleA Comparison of Mushroom Tyrosinase Dopaquinone and Dopachrome Assays Using Diode-Array Spectrophotometry: Dopachrome Formation vs Ascorbate-Linked Dopaquinone Reductionen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelMaterials Science and Engineeringen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Chemistry, University of Michigan, Flint, MI, USA.en_US
dc.contributor.affiliationumDepartment of Chemistry, University of Michigan, Flint, MI, USA.en_US
dc.contributor.affiliationumDepartment of Chemistry, University of Michigan, Flint, MI, USA.en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/30995/1/0000670.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1006/mchj.1993.1040en_US
dc.identifier.sourceMicrochemical Journalen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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