Vasopressin Stimulates DNA Synthesis in Cultured Rat Hepatocytes
dc.contributor.author | Bhora, Faizullah Y. | en_US |
dc.contributor.author | Kothary, Piyush C. | en_US |
dc.contributor.author | Imanishi, Hiroaki | en_US |
dc.contributor.author | Eckhauser, Frederic E. | en_US |
dc.contributor.author | Raper, Steven E. | en_US |
dc.date.accessioned | 2006-04-10T17:44:11Z | |
dc.date.available | 2006-04-10T17:44:11Z | |
dc.date.issued | 1994-12 | en_US |
dc.identifier.citation | Bhora, Faizullah Y., Kothary, Piyush C., Imanishi, Hiroaki, Eckhauser, Frederic E., Raper, Steven E. (1994/12)."Vasopressin Stimulates DNA Synthesis in Cultured Rat Hepatocytes." Journal of Surgical Research 57(6): 706-710. <http://hdl.handle.net/2027.42/31168> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WM6-45P0G1B-D/2/b9cc636c61d0ae2a54800827250ad9bd | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/31168 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7996850&dopt=citation | en_US |
dc.description.abstract | Liver regeneration following partial hepatectomy is significantly impaired in rats with hereditary vasopressin (AVP) deficiency. This suggested that AVP might have a direct effect on cultured rat hepatocytes. Hepatocytes from male Sprague-Dawley rats were isolated using a two-step collaganase perfusion technique and plated at a density of 105/16-mm Primaria plate. After a suitable attachment period, hepatocytes were incubated with minimal essential media, AVP, AVP plus a specific AVP antagonist, or oxytocin. Hepatocyte proliferation was measured by [3H]thymidine incorporation ([3H]Thy) into hepatocyte DNA. AVP (10 nM) increased [3H]Thy significantly (and this effect was blocked by an AVP-specific antagonist (50 nM). Oxytocin had no effect on hepatocyte DNA synthesis. To further investigate the influence of AVP on hepatocyte proliferation, the effect of AVP on transforming growth factor-[alpha] (TGF-[alpha])-stimulated hepatocyte proliferation was also studied. This combination was chosen based on the ability of AVP to inhibit the biologic effects of EGF (a TGF-[alpha] analog). There was significant attenuation of TGF-[alpha] (50 nM)-stimulated [3H]Thy in the presence of AVP (10 nM). In summary: (l) AVP stimulates proliferation of cultured rat hepatocytes. (2) The effect of AVP can be significantly abolished by a specific AVP antagonist. (3) The proliferative response of AVP is specific. (4) AVP significantly attenuates TGF-[alpha]-stimulated hepatocyte hepatic DNA synthesis. Further studies should elucidate the mechanisms for the effects of AVP on hepatic proliferation alone or in combination with other factors. | en_US |
dc.format.extent | 327197 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Vasopressin Stimulates DNA Synthesis in Cultured Rat Hepatocytes | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Surgery and Anesthesiology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan 48109 | en_US |
dc.identifier.pmid | 7996850 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/31168/1/0000067.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1006/jsre.1994.1205 | en_US |
dc.identifier.source | Journal of Surgical Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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