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Lack of correspondence between the room-temperature phosphorescence decay-components and Trp residues in a series of Trp-->Cys or Trp-->Phemutants of human carbonic anhydrase II

dc.contributor.authorBergenhem, Nils C. H.en_US
dc.contributor.authorSchlyer, Bruce D.en_US
dc.contributor.authorSteel, Duncan G.en_US
dc.contributor.authorGafni, Arien_US
dc.contributor.authorCarlsson, Unoen_US
dc.contributor.authorJonsson, Bengt-Haralden_US
dc.date.accessioned2006-04-10T17:49:21Z
dc.date.available2006-04-10T17:49:21Z
dc.date.issued1994-10-17en_US
dc.identifier.citationBergenhem, Nils C. H., Schlyer, Bruce D., Steel, Duncan G., Gafni, Ari, Carlsson, Uno, Jonsson, Bengt-Harald (1994/10/17)."Lack of correspondence between the room-temperature phosphorescence decay-components and Trp residues in a series of Trp--&gt;Cys or Trp--&gt;Phemutants of human carbonic anhydrase II." FEBS Letters 353(2): 177-179. <http://hdl.handle.net/2027.42/31251>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T36-447MX41-4X/2/00d07af8c24f10c0096dc37c7f6ff813en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/31251
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7926047&dopt=citationen_US
dc.description.abstractThe room temperature phosphorescence of native human carbonic anhydrase (CA), and several mutants of this enzyme has been investigated. In these mutants the seven tryptophan residues in the native protein have sequentially been replaced by cysteine or phenylalanine. All of the mutants as well as native CA show room-temperature tryptophan phosphorescence (RTP) spectra. Surprisingly, only small differences in RTP life-times are noticeable among these mutants, indicating that there is more than one tryptophan residue with similar phosphorescence decay kinetics in the protein. The present results illustrate the danger in attributing the room temperature phosphorescence of a multi-tryptophan protein to a particular residue based solely on an analysis of the protein structure.en_US
dc.format.extent280183 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleLack of correspondence between the room-temperature phosphorescence decay-components and Trp residues in a series of Trp--&#62;Cys or Trp--&#62;Phemutants of human carbonic anhydrase IIen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumInstitute of Gerontology, University of Michigan, Ann Arbor, MI 48109-2007, USAen_US
dc.contributor.affiliationumInstitute of Gerontology, University of Michigan, Ann Arbor, MI 48109-2007, USA; Department of Biological Chemistry University of Michigan, Ann Arbor, MI 48109-2007, USA.en_US
dc.contributor.affiliationumDepartment of Physics and Electrical Engineering, University of Michigan, Ann Arbor, MI 48109-2007, USA; Institute of Gerontology, University of Michigan, Ann Arbor, MI 48109-2007, USA.en_US
dc.contributor.affiliationumDepartment of Biological Chemistry University of Michigan, Ann Arbor, MI 48109-2007, USA; Institute of Gerontology, University of Michigan, Ann Arbor, MI 48109-2007, USA.en_US
dc.contributor.affiliationotherIFM/Department of Chemistry, Linköping University, S-581 83 Linköping, Swedenen_US
dc.contributor.affiliationotherDepartment of Biochemistry, Umeå University, S-901 87, Umeå, Swedenen_US
dc.identifier.pmid7926047en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/31251/1/0000157.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0014-5793(94)01041-2en_US
dc.identifier.sourceFEBS Lettersen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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