Hydrogenation of testosterone sulfate by the [Delta]4-3-ketosteroid reductase of rat liver microsomes

Abstract

TPNH2 is oxidized in the presence of testosterone or testosterone sulfate and a microsomal preparation from the liver of female rats. In the presence of iorganic phosphate, DPNH2 also acts as a hydrogen donor. Evidence is presented that testosterone sulfate is converted predominantly to the 4,5-dihydro derivative, 3-keto-5[alpha]-androstane - 17-yl sulfate.