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The photometric estimation of luciferase in luminous bacterial cells

dc.contributor.authorBurns, Charles H.en_US
dc.contributor.authorNevill, A. D.en_US
dc.contributor.authorWhipple, G. Hoyten_US
dc.date.accessioned2006-04-13T14:51:35Z
dc.date.available2006-04-13T14:51:35Z
dc.date.issued1963-05en_US
dc.identifier.citationBurns, Charles H., Nevill, A. D., Whipple, G. Hoyt (1963/05)."The photometric estimation of luciferase in luminous bacterial cells." Analytical Biochemistry 5(5): 393-415. <http://hdl.handle.net/2027.42/32226>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6W9V-4DVNKHY-M/2/09b9b30d6233b3befbe6f61e44f09037en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/32226
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=14017149&dopt=citationen_US
dc.description.abstractThe bacterial luciferase reaction carried out in vitro can be used as a basis for a semiquantitative assay of active luciferase in bacterial cells. Relations between maximum luminous intensity generated and the amount of enzyme present can be established for luciferase and for the combination of DPNH oixdase and luciferase. A definition is suggested for a unit of luciferase activity. It is believed that the assay procedure described can be made accurate to within +/-10%.en_US
dc.format.extent1324517 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleThe photometric estimation of luciferase in luminous bacterial cellsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumSchool of Public Health, The University of Michigan, Ann Arbor, Michigan, USAen_US
dc.contributor.affiliationumSchool of Public Health, The University of Michigan, Ann Arbor, Michigan, USAen_US
dc.contributor.affiliationumSchool of Public Health, The University of Michigan, Ann Arbor, Michigan, USAen_US
dc.identifier.pmid14017149en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/32226/1/0000286.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0003-2697(63)90041-1en_US
dc.identifier.sourceAnalytical Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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