Alginate type and RGD density control myoblast phenotype
dc.contributor.author | Rowley, Jon A. | en_US |
dc.contributor.author | Mooney, David J. | en_US |
dc.date.accessioned | 2006-04-19T13:33:42Z | |
dc.date.available | 2006-04-19T13:33:42Z | |
dc.date.issued | 2002-05 | en_US |
dc.identifier.citation | Rowley, Jon A.; Mooney, David J. (2002)."Alginate type and RGD density control myoblast phenotype." Journal of Biomedical Materials Research 60(2): 217-223. <http://hdl.handle.net/2027.42/34424> | en_US |
dc.identifier.issn | 0021-9304 | en_US |
dc.identifier.issn | 1097-4636 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34424 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=11857427&dopt=citation | en_US |
dc.description.abstract | Alginates are being increasingly used for cell encapsulation and tissue engineering applications; however, these materials cannot specifically interact with mammalian cells. We have covalently modified alginates of varying monomeric ratio with RGD-containing cell adhesion ligands using carbodiimide chemistry to initiate cell adhesion to these polymers. We hypothesized that we could control the function of cells adherent to RGD-modified alginate hydrogels by varying alginate polymer type and cell adhesion ligand density, and we have addressed this possibility by studying the proliferation and differentiation of C2C12 skeletal myoblasts adherent to these materials. RGD density on alginates of varying monomeric ratio could be controlled over several orders of magnitude, creating a range of surface densities from 1–100 fmol/cm 2 . Myoblast adhesion to these materials was specific to the RGD ligand, because adhesion could be competed away with soluble RGD in a dose-dependent manner. Myoblast proliferation and differentiation could be regulated by varying the alginate monomeric ratio and the density of RGD ligands at the substrate surface, and specific combinations of alginate type and RGD density were required to obtain efficient myoblast differentiation on these materials. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 60: 217–223, 2002; DOI 10.1002/jbm.1287 | en_US |
dc.format.extent | 4179750 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Polymer and Materials Science | en_US |
dc.title | Alginate type and RGD density control myoblast phenotype | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Biomedical Engineering | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biomedical Engineering, University of Michigan, Colleges of Engineering and Dentistry, Ann Arbor, Michigan ; Department of Biomedical Engineering, University of Michigan, Colleges of Engineering and Dentistry, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Biomedical Engineering, University of Michigan, Colleges of Engineering and Dentistry, Ann Arbor, Michigan ; Department of Chemical Engineering, University of Michigan, Colleges of Engineering and Dentistry, Ann Arbor, Michigan ; Department of Biologic and Materials Science, University of Michigan, Colleges of Engineering and Dentistry, Ann Arbor, Michigan | en_US |
dc.identifier.pmid | 11857427 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34424/1/1287_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/jbm.1287 | en_US |
dc.identifier.source | Journal of Biomedical Materials Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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