Induction of α1-tubulin gene expression during development and regeneration of the fish central nervous system
dc.contributor.author | Hieber, Virginia C. | en_US |
dc.contributor.author | Dai, Xinhua | en_US |
dc.contributor.author | Foreman, Mark | en_US |
dc.contributor.author | Goldman, Daniel J. | en_US |
dc.date.accessioned | 2006-04-19T13:36:13Z | |
dc.date.available | 2006-04-19T13:36:13Z | |
dc.date.issued | 1998-11-15 | en_US |
dc.identifier.citation | Hieber, Virginia; Dai, Xinhua; Foreman, Mark; Goldman, Daniel (1998)."Induction of α1-tubulin gene expression during development and regeneration of the fish central nervous system." Journal of Neurobiology 37(3): 429-440. <http://hdl.handle.net/2027.42/34478> | en_US |
dc.identifier.issn | 0022-3034 | en_US |
dc.identifier.issn | 1097-4695 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/34478 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9828048&dopt=citation | en_US |
dc.description.abstract | The α1- and α2-tubulin encoding genes were cloned from a goldfish genomic DNA library. α1- and α2-tubulin RNA expression was examined in developing and adult retinas. These studies demonstrated increased α1-tubulin RNA in presumptive ganglion cells that grow axons early in retinal development and in adult retinal ganglion cells whose optic axons had been damaged. The α2-tubulin RNA was undetectable in developing retina and constitutively expressed in adult retinal ganglion cells regardless of optic nerve crush. To determine if these changes in α1-tubulin RNA reflected changes in α1-tubulin promoter activity, we introduced into zebrafish embryos and adult goldfish retinal explants expression vectors harboring the α1-tubulin gene's promoter. These studies showed that the α1-tubulin promoter confers a developmentally regulated, neuron-restricted pattern of reporter gene expression in vivo and its activity is increased in adult retinal neurons induced to regenerate their axons. Promoter deletions defined regions of α1-tubulin DNA necessary for this pattern of expression. These results suggest that DNA sequences necessary for α1-tubulin gene induction during central nervous system development and regeneration are contained within the α1-tubulin gene's 5′-flanking DNA and that this promoter will be useful for identifying these elements and their DNA binding proteins. © 1998 John Wiley & Sons, Inc. J Neurobiol 37: 429–440, 1998 | en_US |
dc.format.extent | 496021 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Neuroscience, Neurology and Psychiatry | en_US |
dc.title | Induction of α1-tubulin gene expression during development and regeneration of the fish central nervous system | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute and Department of Biological Chemistry, 205 Zina Pitcher Place, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Mental Health Research Institute and Department of Biological Chemistry, 205 Zina Pitcher Place, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Mental Health Research Institute and Department of Biological Chemistry, 205 Zina Pitcher Place, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Mental Health Research Institute and Department of Biological Chemistry, 205 Zina Pitcher Place, University of Michigan, Ann Arbor, Michigan 48109 ; Mental Health Research Institute and Department of Biological Chemistry, 205 Zina Pitcher Place, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.identifier.pmid | 9828048 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/34478/1/8_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/(SICI)1097-4695(19981115)37:3<429::AID-NEU8>3.0.CO;2-N | en_US |
dc.identifier.source | Journal of Neurobiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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