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Immobilization can improve the stability of hybridoma antibody productivity in serum-free media

dc.contributor.authorLee, Gyun Minen_US
dc.contributor.authorPalsson, Bernhard Øen_US
dc.date.accessioned2006-04-28T16:30:22Z
dc.date.available2006-04-28T16:30:22Z
dc.date.issued1990-12-05en_US
dc.identifier.citationLee, Gyun Min; Palsson, Bernhard O. (1990)."Immobilization can improve the stability of hybridoma antibody productivity in serum-free media." Biotechnology and Bioengineering 36(10): 1049-1055. <http://hdl.handle.net/2027.42/37905>en_US
dc.identifier.issn0006-3592en_US
dc.identifier.issn1097-0290en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/37905
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18595043&dopt=citationen_US
dc.description.abstractHybridoma cells (S3H5/Γ2bA2) were cultivated in spinner flasks with 1% serum media and serum-free media. Monoclonal antibody productivity was maintained in 1% serum media. However, cells in serum-free media showed a decrease in antibody productivity, and it completely disappeared in IMDM-based low protein medium. This loss of antibody productivity was not observed when the cells were immobilized in alginate beads. In fact, immobilization enhanced the specific MAb productivity.en_US
dc.format.extent684563 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherWiley Subscription Services, Inc., A Wiley Companyen_US
dc.subject.otherChemistryen_US
dc.subject.otherBiochemistry and Biotechnologyen_US
dc.titleImmobilization can improve the stability of hybridoma antibody productivity in serum-free mediaen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbsecondlevelMathematicsen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelStatistics and Numeric Dataen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelSocial Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, Herbert H. Dow Building, Ann Arbor, Michigan 48109en_US
dc.contributor.affiliationumDepartment of Chemical Engineering, University of Michigan, Herbert H. Dow Building, Ann Arbor, Michigan 48109 ; Department of Chemical Engineering, University of Michigan, Herbert H. Dow Building, Ann Arbor, Michigan 48109en_US
dc.identifier.pmid18595043en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/37905/1/260361010_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/bit.260361010en_US
dc.identifier.sourceBiotechnology and Bioengineeringen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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