Irreversible enzyme-shuttle immunoassay
dc.contributor.author | Paek, Se-Hwan | en_US |
dc.contributor.author | Schramm, Willfried | en_US |
dc.date.accessioned | 2006-04-28T16:31:10Z | |
dc.date.available | 2006-04-28T16:31:10Z | |
dc.date.issued | 1992-03-25 | en_US |
dc.identifier.citation | Paek, Se-Hwan; Schramm, Willfried (1992)."Irreversible enzyme-shuttle immunoassay." Biotechnology and Bioengineering 39(7): 753-764. <http://hdl.handle.net/2027.42/37921> | en_US |
dc.identifier.issn | 0006-3592 | en_US |
dc.identifier.issn | 1097-0290 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/37921 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18601007&dopt=citation | en_US |
dc.description.abstract | The concept of a competitive enzyme immunoassay that utilizes simultaneously the bound and the free analyte-enzyme conjugate (heterobifunctional conjugate) for signal generation in response to varying analyte concentrations in samples has been investigated. Two antigenic sites of the heterobifunctional conjugate are used in the assay for binding to immunoglobulins: the analyte derivative binds to an immobilized antibody, Ab 1 , and the enzyme component binds to a spatially separated immobilized antibody, Ab 2 . The analytical system is set up such that in the absence of analyte, the conjugate is predominantly bound in the compartment that contains Ab 1 . With increasing concentration of native analyte in samples, an increasing concentration of native analyte in samples, an increasing amount of conjugate migrates to the second compartment that contains Ab 2 . The enzyme bound in each compartment is used for signal generation. Mathematical models have been developed to determine the optimal conditions and to predict the performance of such dual-antibody systems. The theoretical predictions are supported by experimental results. The dual-antibody system has been compared with a conventional competitive enzyme immunoassay using the same reagents. | en_US |
dc.format.extent | 1191626 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Biochemistry and Biotechnology | en_US |
dc.title | Irreversible enzyme-shuttle immunoassay | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Mathematics | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Statistics and Numeric Data | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan, Reproductive Sciences Program and Bioengineering Program, 300 N. Ingalls, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | University of Michigan, Reproductive Sciences Program; and BioQuant, Inc., 1919 Green Rd., Ann Arbor, Michigan 48105 ; University of Michigan, Reproductive Sciences Program; and BioQuant, Inc., 1919 Green Rd., Ann Arbor, Michigan 48105 | en_US |
dc.identifier.pmid | 18601007 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/37921/1/260390708_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/bit.260390708 | en_US |
dc.identifier.source | Biotechnology and Bioengineering | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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