Uptake of metal cations by fibroblasts in vitro
dc.contributor.author | Wataha, John C. | en_US |
dc.contributor.author | Hanks, Carl T. | en_US |
dc.contributor.author | Craig, Robert G. | en_US |
dc.date.accessioned | 2006-04-28T16:35:17Z | |
dc.date.available | 2006-04-28T16:35:17Z | |
dc.date.issued | 1993-02 | en_US |
dc.identifier.citation | Wataha, J. C.; Hanks, C. T.; Craig, R. G. (1993)."Uptake of metal cations by fibroblasts in vitro ." Journal of Biomedical Materials Research 27(2): 227-232. <http://hdl.handle.net/2027.42/38002> | en_US |
dc.identifier.issn | 0021-9304 | en_US |
dc.identifier.issn | 1097-4636 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/38002 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8436579&dopt=citation | en_US |
dc.description.abstract | Atomic absorption spectroscopy was used to assess uptake of Ag +1 , Au +4 , Cd +2 , Cu +2 , In +3 , Ni +2 , Pd +2 , and Zn +2 by in vitro cultures of Balb/c 3T3 fibroblasts as a function of concentration of the cations in cell-culture medium. Reversibility of this uptake was also measured. Metal cations exhibited a 400-fold difference in their tendency to accumulate in the cells; In +3 tended to accumulate the most, whereas Pd +2 accumulated the least. Uptake of the cations in the cells increased linearly with the concentration of the cation in the medium for all cations up to their 50% toxicity concentrations. Reversal of this uptake was slower than that of the initial uptake for three cations studied in more detail (Cd +2 , In +3 , and Ni +2 ). The duration of the initial exposure affected the proportion of the metal cations that were retained by the cells 5 h after the cations were removed from the medium. The proportion of retained Cd +2 did not change when the initial exposure was increased from 2 h to 6 h, whereas the proportion of retained In +3 decreased and Ni +2 increased over the same period. The tendency of the cells to accumulate these cations correlated with their cytotoxic potency (measured previously). © 1993 John Wiley & Sons, Inc. | en_US |
dc.format.extent | 671638 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | John Wiley & Sons, Inc. | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Polymer and Materials Science | en_US |
dc.title | Uptake of metal cations by fibroblasts in vitro | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Biomedical Engineering | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | The University of Michigan School of Dentistry, Ann Arbor, Michigan 48109–1078 ; The University of Michigan School of Dentistry, Ann Arbor, Michigan 48109–1078 | en_US |
dc.contributor.affiliationum | The University of Michigan School of Dentistry, Ann Arbor, Michigan 48109–1078 | en_US |
dc.contributor.affiliationum | The University of Michigan School of Dentistry, Ann Arbor, Michigan 48109–1078 | en_US |
dc.identifier.pmid | 8436579 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/38002/1/820270212_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/jbm.820270212 | en_US |
dc.identifier.source | Journal of Biomedical Materials Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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