Role of the neural retina in newt ( Notophthalmus viridescens ) lens regeneration in vitro

Abstract

Removal of the lens from the eye of an adult newt ( Notophthalmus viridescens ) is followed by regeneration of a new lens from the dorsal iris epithelial cells at the pupillary margin. This process is dependent upon the neural retina for its normal completion in vivo and in vitro. To examine the relationship between the retina and lens regeneration, we have conducted experiments that delimit the time period during which the retinal presence is critical (in vivo) and have investigated the influence of extracts of the retina on the progress of regeneration (in vitro). In vivo, removal of the retina at day 11 seriously retards further progression of regeneration while removal of the retina at day 15 does not retard regeneration significantly. This defines a “critical period” in regeneration of the lens during which the retina is required. Explantation of regenerates 11 or 12 days after lentectomy to organ culture medium enriched with either crude retinal homogenate or extracts prepared from chick or bovine retinas according to Courty et al. ('85, Biochimie, 67: 265–269) reveals that the progress of regeneration can be supported in culture by the crude extract. This is the first demonstration of complete iris-lens trnsformation in culture in the presence of retinal extract. It is possible that the retina acts indirectly by promoting passage of the iris epithelial cells through the critical number of mitoses required before redifferentiation into lens cells can occur (as proposed by Yamada, '77, Monogr. Dev. Biol., 13: 126). It is also possible that the retina acts by directly instructing the iris cells to redifferentiate. Our experiments provide some indirect support for the first possibility but do not distinguish between them at this time.