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Transport of amino acids in intact 3T3 and SV3T3 cells. Binding activity for leucine in membrane preparations of ehrlich ascites tumor cells

dc.contributor.authorCecchini, Garyen_US
dc.contributor.authorLee, Maryen_US
dc.contributor.authorOxender, Dale L.en_US
dc.date.accessioned2006-04-28T16:45:30Z
dc.date.available2006-04-28T16:45:30Z
dc.date.issued1976en_US
dc.identifier.citationCecchini, Gary; Lee, Mary; Oxender, Dale L. (1976)."Transport of amino acids in intact 3T3 and SV3T3 cells. Binding activity for leucine in membrane preparations of ehrlich ascites tumor cells." Journal of Supramolecular Structure 4(4): 441-447. <http://hdl.handle.net/2027.42/38202>en_US
dc.identifier.issn0091-7419en_US
dc.identifier.issn1547-9366en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/38202
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=180354&dopt=citationen_US
dc.description.abstractTransport of amino acids into 3T3 and SV3T3 (SV40 virus-transformed 3T3) cells was measured on glass cover slips. The 3T3 and SV3T3 cells contain both A (alanine preferring) and L (leucine preferring) systems for neutral amino acid transport. Initial rates of uptake of amino acids are about twofold higher in SV3T3 than in 3T3 cells. Other parameters measured, however, do not indicate marked differences in the transport of amino acids by the two cell types. L-system amino acids, such as leucine, are subject to trans-stimulation in both cell lines, whereas A-system amino acids, such as alanine and glycine, are not. Leucine was transported to higher levels in confluent cells than in nonconfluent cells. Glycine, however, shows distinctly less transport activity as the cells become confluent. Ehrlich ascites cell plasma membranes were prepared and assayed for amino acid-binding activity. Leucine-binding activity was detected by equilibrium dialysis in Triton X-100-treated membrane preparations.en_US
dc.format.extent325060 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherAlan R. Liss, Inc.en_US
dc.publisherWiley Periodiocals, Inc.en_US
dc.subject.otherLife Sciencesen_US
dc.subject.otherMolecular Cell Biologyen_US
dc.titleTransport of amino acids in intact 3T3 and SV3T3 cells. Binding activity for leucine in membrane preparations of ehrlich ascites tumor cellsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, Medical Science I, The University of Michigan, Ann Arbor, Michiganen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, Medical Science I, The University of Michigan, Ann Arbor, Michiganen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, Medical Science I, The University of Michigan, Ann Arbor, Michiganen_US
dc.identifier.pmid180354en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/38202/1/400040403_ftp.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1002/jss.400040403en_US
dc.identifier.sourceJournal of Supramolecular Structureen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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