Transport of amino acids in intact 3T3 and SV3T3 cells. Binding activity for leucine in membrane preparations of ehrlich ascites tumor cells
dc.contributor.author | Cecchini, Gary | en_US |
dc.contributor.author | Lee, Mary | en_US |
dc.contributor.author | Oxender, Dale L. | en_US |
dc.date.accessioned | 2006-04-28T16:45:30Z | |
dc.date.available | 2006-04-28T16:45:30Z | |
dc.date.issued | 1976 | en_US |
dc.identifier.citation | Cecchini, Gary; Lee, Mary; Oxender, Dale L. (1976)."Transport of amino acids in intact 3T3 and SV3T3 cells. Binding activity for leucine in membrane preparations of ehrlich ascites tumor cells." Journal of Supramolecular Structure 4(4): 441-447. <http://hdl.handle.net/2027.42/38202> | en_US |
dc.identifier.issn | 0091-7419 | en_US |
dc.identifier.issn | 1547-9366 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/38202 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=180354&dopt=citation | en_US |
dc.description.abstract | Transport of amino acids into 3T3 and SV3T3 (SV40 virus-transformed 3T3) cells was measured on glass cover slips. The 3T3 and SV3T3 cells contain both A (alanine preferring) and L (leucine preferring) systems for neutral amino acid transport. Initial rates of uptake of amino acids are about twofold higher in SV3T3 than in 3T3 cells. Other parameters measured, however, do not indicate marked differences in the transport of amino acids by the two cell types. L-system amino acids, such as leucine, are subject to trans-stimulation in both cell lines, whereas A-system amino acids, such as alanine and glycine, are not. Leucine was transported to higher levels in confluent cells than in nonconfluent cells. Glycine, however, shows distinctly less transport activity as the cells become confluent. Ehrlich ascites cell plasma membranes were prepared and assayed for amino acid-binding activity. Leucine-binding activity was detected by equilibrium dialysis in Triton X-100-treated membrane preparations. | en_US |
dc.format.extent | 325060 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Alan R. Liss, Inc. | en_US |
dc.publisher | Wiley Periodiocals, Inc. | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Molecular Cell Biology | en_US |
dc.title | Transport of amino acids in intact 3T3 and SV3T3 cells. Binding activity for leucine in membrane preparations of ehrlich ascites tumor cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, Medical Science I, The University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, Medical Science I, The University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, Medical Science I, The University of Michigan, Ann Arbor, Michigan | en_US |
dc.identifier.pmid | 180354 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/38202/1/400040403_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/jss.400040403 | en_US |
dc.identifier.source | Journal of Supramolecular Structure | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.