Restriction fragment length polymorphisms distinguish among accessions of Ceratopteris thalictroides and C. richardii ( Parkeriaceae )
dc.contributor.author | Hickok, Leslie G. | en_US |
dc.contributor.author | McGrath, J. Mitchell | en_US |
dc.contributor.author | Pichersky, Eran | en_US |
dc.date.accessioned | 2006-09-08T19:28:30Z | |
dc.date.available | 2006-09-08T19:28:30Z | |
dc.date.issued | 1994-09 | en_US |
dc.identifier.citation | McGrath, J. Mitchell; Hickok, Leslie G.; Pichersky, Eran; (1994). "Restriction fragment length polymorphisms distinguish among accessions of Ceratopteris thalictroides and C. richardii ( Parkeriaceae )." Plant Systematics and Evolution 189 (3-4): 193-202. <http://hdl.handle.net/2027.42/41637> | en_US |
dc.identifier.issn | 1615-6110 | en_US |
dc.identifier.issn | 0378-2697 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/41637 | |
dc.description.abstract | We have used cDNA clones as probes on Southern blots to detect restriction fragment length polymorphisms among seven Ceratopteris thalictroides accessions, three C. richardii accessions, and one putative interspecific hybrid. We found that the stringency of post-hybridization washes was a critical parameter affecting the quality of our blots; even with homologous cDNA sequences low stringency conditions resulted in a smear of signal, but high stringency washes gave blots with distinct bands. Most probes showed hybridization with four or more genomic fragments. Similarities in the number and size of fragments between and within species indicated that (i) C. richardii shows limited polymorphism among accessions tested, (ii) C. thalictroides is highly polymorphic, and (iii) Hawaiian accessions of C. thalictroides are divergent relative to their continental cohorts and among themselves. The putative interspecific hybrid did not group closely with either of these species. | en_US |
dc.format.extent | 1030751 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Polyploidy | en_US |
dc.subject.other | RFLP | en_US |
dc.subject.other | Plant Ecology | en_US |
dc.subject.other | CDNA | en_US |
dc.subject.other | Homosporous Ferns | en_US |
dc.subject.other | Ceratopteris | en_US |
dc.subject.other | Plant Anatomy/Development | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | C. Richardii | en_US |
dc.subject.other | Plant Systematics/Taxonomy/Biogeography | en_US |
dc.subject.other | Parkeriaceae | en_US |
dc.subject.other | Plant Sciences | en_US |
dc.subject.other | C. Thalictroides | en_US |
dc.title | Restriction fragment length polymorphisms distinguish among accessions of Ceratopteris thalictroides and C. richardii ( Parkeriaceae ) | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biology, University of Michigan, 48109-1048, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Department of Plant Pathology, University of Wisconsin, 1630 Linden Dr., 53706, Madison, WI, USA | en_US |
dc.contributor.affiliationother | Department of Botany, University of Tennessee, 37996, Knoxville, TN, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/41637/1/606_2004_Article_BF00939725.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00939725 | en_US |
dc.identifier.source | Plant Systematics and Evolution | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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