Mucin in middle ear effusions inhibits attachment of Haemophilus influenzae to mucosal epithelial cells
dc.contributor.author | van Alphen, Loek | en_US |
dc.contributor.author | Gilsdorf, Janet R. | en_US |
dc.contributor.author | Hanisch, Franz-Georg | en_US |
dc.contributor.author | Schroten, Horst | en_US |
dc.contributor.author | Solzbacher, Daniela | en_US |
dc.date.accessioned | 2006-09-08T20:05:45Z | |
dc.date.available | 2006-09-08T20:05:45Z | |
dc.date.issued | 2003-03 | en_US |
dc.identifier.citation | Solzbacher, Daniela; Hanisch, Franz-Georg; van Alphen, Loek; Gilsdorf, Janet R.; Schroten, Horst; (2003). "Mucin in middle ear effusions inhibits attachment of Haemophilus influenzae to mucosal epithelial cells." European Archives of Oto-Rhino-Laryngology 260(3): 141-147. <http://hdl.handle.net/2027.42/42217> | en_US |
dc.identifier.issn | 0937-4477 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42217 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=12687386&dopt=citation | en_US |
dc.description.abstract | Although otitis media with effusion is often preceded by an infection of the tympanic cavity, when cultured, many effusions show no culturable bacteria. Based on the hypothesis that the effusion might play a protective role in the course of infection, the influence of this fluid on adhesion of H. influenzae (Hi) type-b strain 770235 and nontypeable H. influenaze (NTHi) strains to buccal epithelial cells was investigated. Effusions were classified as mucoid, seromucoid and serous. Mucoid secretions inhibited adhesion to a significantly greater extent (62%) than did seromucous (52%) and serous effusions (47%) ( P <0.001). The glycoprotein and high-molecular-weight fractions showed similar levels of inhibition. Sialic acid concentration, and, to a lesser extent, protein concentration, correlated with the level of inhibition. Desialylated effusions lost their ability to block bacterial attachment. Thus, middle ear effusion fluid exhibits an inhibitory effect that is due to mucins, which determine viscosity and represent the sialylated high-molecular-weight glycoprotein fraction of the effusion. | en_US |
dc.format.extent | 251863 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Mucin Bacterial Adhesion Otitis Media | en_US |
dc.subject.other | Legacy | en_US |
dc.title | Mucin in middle ear effusions inhibits attachment of Haemophilus influenzae to mucosal epithelial cells | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Otolaryngology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pediatrics, University of Michigan Medical School, Ann Arbor, Michigan, USA, | en_US |
dc.contributor.affiliationother | Institute of Biochemistry, Medical Faculty, University of Cologne, Cologne, Germany, | en_US |
dc.contributor.affiliationother | Department of Otorhinolaryngology, University of Essen, Essen, Germany, | en_US |
dc.contributor.affiliationother | University Children's Hospital, Moorenstr. 5, 40225 Düsseldorf, Germany, | en_US |
dc.contributor.affiliationother | National Institute for Public Health and the Environment, Bilthoven, The Netherlands, | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 12687386 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42217/1/s00405-002-0535-1.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s00405-002-0535-1 | en_US |
dc.identifier.source | European Archives of Oto-Rhino-Laryngology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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