A numerical simulation of trichromatic equations in chlorophyll estimation using the spectrophotometric technique

Abstract

A numerical simulation of trichromatic pigment equations is made with the aid of a computer utility program. Significant quantitative differences in the estimates of pigment concentration result from using different sets of trichromatic equations. Estimates of chlorophylls a, b , and c were found highly correlated with the application of the equations, even though the absorbance values used as input for the stimulation are not correlated.