A triple-resonance pulse scheme for selectively correlating amide 1 H N and 15 N nuclei with the 1 H α proton of the preceding residue
dc.contributor.author | Clubb, Robert T. | en_US |
dc.contributor.author | Wagner, Gerhard | en_US |
dc.date.accessioned | 2006-09-08T21:00:24Z | |
dc.date.available | 2006-09-08T21:00:24Z | |
dc.date.issued | 1992-07 | en_US |
dc.identifier.citation | Clubb, Robert T.; Wagner, Gerhard; (1992). "A triple-resonance pulse scheme for selectively correlating amide 1 H N and 15 N nuclei with the 1 H α proton of the preceding residue." Journal of Biomolecular NMR 2(4): 389-394. <http://hdl.handle.net/2027.42/43044> | en_US |
dc.identifier.issn | 0925-2738 | en_US |
dc.identifier.issn | 1573-5001 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/43044 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1324757&dopt=citation | en_US |
dc.description.abstract | A 3D 1 H− 15 N− 13 C triple resonance experiment is presented that contains exclusively cross peaks between the 1 H N and 15 N nuclei of one residue with the H α of the preceding residue. The pulse sequence, designed to minimize the time coherence, is transverse on nuclei with short T 2 values. The experiment consists of coherence transfers via one-bond couplings from the H N via N, CO, C α to the H α and back to the H N for detection; it is called HN(COCA)HA. The experiment was tested on uniformly 15 N- and 13 C-enriched T4 lysozyme. | en_US |
dc.format.extent | 304739 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; ESCOM Science Publishers B.V. ; Springer Science+Business Media | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Polymer Sciences | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Biophysics/Biomedical Physics | en_US |
dc.subject.other | 3D NMR | en_US |
dc.subject.other | Triple Resonance | en_US |
dc.subject.other | J Connectivity | en_US |
dc.subject.other | Resonance Assignment | en_US |
dc.subject.other | Isotope Labeling | en_US |
dc.subject.other | Proteins | en_US |
dc.subject.other | T4 Lysozyme | en_US |
dc.title | A triple-resonance pulse scheme for selectively correlating amide 1 H N and 15 N nuclei with the 1 H α proton of the preceding residue | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, 02115, Boston, MA, USA; Department of Biological Chemistry, University of Michigan, 1301 Catherine Street, M148109, Ann Arbor, USA | en_US |
dc.contributor.affiliationother | Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, 02115, Boston, MA, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 1324757 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/43044/1/10858_2005_Article_BF01874816.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF01874816 | en_US |
dc.identifier.source | Journal of Biomolecular NMR | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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