A method for the isolation of human gastric mucous epithelial cells for primary cell culture: A comparison of biopsy vs surgical tissue
dc.contributor.author | Rutten, Michael J. | en_US |
dc.contributor.author | Campbell, Donald R. | en_US |
dc.contributor.author | Luttropp, Cheryl A. | en_US |
dc.contributor.author | Fowler, Wendy M. | en_US |
dc.contributor.author | Hawkey, Mitchell A. | en_US |
dc.contributor.author | Boland, C. Richard | en_US |
dc.contributor.author | Kraus, Eugene R. | en_US |
dc.contributor.author | Sheppard, Brett C. | en_US |
dc.contributor.author | Crass, Richard A. | en_US |
dc.contributor.author | Deeveney, Karen E. | en_US |
dc.contributor.author | Deveney, Clifford W. | en_US |
dc.date.accessioned | 2006-09-08T21:13:02Z | |
dc.date.available | 2006-09-08T21:13:02Z | |
dc.date.issued | 1996-12 | en_US |
dc.identifier.citation | Rutten, Michael J.; Campbell, Donald R.; Luttropp, Cheryl A.; Fowler, Wendy M.; Hawkey, Mitchell A.; Boland, C. Richard; Kraus, Eugene R.; Sheppard, Brett C.; Crass, Richard A.; Deeveney, Karen E.; Deveney, Clifford W.; (1996). "A method for the isolation of human gastric mucous epithelial cells for primary cell culture: A comparison of biopsy vs surgical tissue." Methods in Cell Science 18(4): 269-281. <http://hdl.handle.net/2027.42/43235> | en_US |
dc.identifier.issn | 1381-5741 | en_US |
dc.identifier.issn | 1573-0603 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/43235 | |
dc.description.abstract | We have developed a method for the isolation and growth of normal human gastric mucous epithelial cells using biopsies or surgically resected tissues as the source of the cells. The attachment and growth of cells were dependent upon: (1) cell planting density, ∼50,000 cells/cm 2 ; (2) extracellular matrix (fibronectin); and (3) and the use of a porous filter. In all experiments we found better cells attachment and growth of human gastric mucous cells isolated from surgical specimens compared with those gastric mucous cells isolated from gastric biopsies. The initial cell viability (as measured by Trypan-blue) was the same in both populations of gastric mucous epithelial cells isolated from either gastric biopsies or surgical specimens. After 4–5 days in culture one could detect various amounts of mucin in all the cells using either periodic acid Schiff (PAS) staining or a specific anti-mucin antibody. A similar pattern of much straining was also found in primary cultures of guinea pig gastric mucous epithelial cells. Immunohistochemical staining for chief cells (anti-pepsinogen) or parietal cells (anti-H + /K + ATPasc) in the gastric mucous cuboidal-like epithelial cells with tight junctions, desmosomes,short microvilli, a filamentous terminal web, mucous granules, and basal lamina-like structure. We could not detect the presence of fibroblasts during the 7–9 days that the primary cells were in culture. This cell culture method will prove useful in the isolation of normal human gastric mucous epithelial cells for in vitro studies of gastric mucosal injury and repair. | en_US |
dc.format.extent | 2989641 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Media | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Animal Biochemistry | en_US |
dc.subject.other | Plant Sciences | en_US |
dc.subject.other | Animal Anatomy / Morphology / Histology | en_US |
dc.subject.other | Cell Culture | en_US |
dc.subject.other | Gastric Mucosa | en_US |
dc.subject.other | Human Cells | en_US |
dc.subject.other | Mucous Epithelial Cells | en_US |
dc.subject.other | Stomach | en_US |
dc.title | A method for the isolation of human gastric mucous epithelial cells for primary cell culture: A comparison of biopsy vs surgical tissue | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Medicine, University of Michigan Medical School, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Department of Medicine, University of Michigan Medical School, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationother | VA Medical Center, Portland, Oregon; Department of Surgery, Oregon Health Sciences University, Portland, Oregon; Department of Surgery-L223A, 3181 Sam Jackson Park Road, 97201, Portland, OR, USA | en_US |
dc.contributor.affiliationother | VA Medical Center, Kansas City; Department of Medicine, University of Kansas Medical Center, Kansas City | en_US |
dc.contributor.affiliationother | VA Medical Center, Portland, Oregon; VA Medical Center, Kansas City | en_US |
dc.contributor.affiliationother | VA Medical Center, Portland, Oregon; Department of Surgery, Oregon Health Sciences University, Portland, Oregon | en_US |
dc.contributor.affiliationother | VA Medical Center, Portland, Oregon; Department of Surgery, Oregon Health Sciences University, Portland, Oregon | en_US |
dc.contributor.affiliationother | VA Medical Center, Portland, Oregon; Department of Surgery, Oregon Health Sciences University, Portland, Oregon | en_US |
dc.contributor.affiliationother | VA Medical Center, Portland, Oregon; Department of Surgery, Oregon Health Sciences University, Portland, Oregon | en_US |
dc.contributor.affiliationother | VA Medical Center, Portland, Oregon; Department of Surgery, Oregon Health Sciences University, Portland, Oregon | en_US |
dc.contributor.affiliationother | VA Medical Center, Portland, Oregon; Department of Surgery, Oregon Health Sciences University, Portland, Oregon | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/43235/1/11022_2004_Article_BF00127904.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00127904 | en_US |
dc.identifier.source | Methods in Cell Science | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.