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Whole-cell patch-clamp recordings from identified spinal neurons in the zebrafish embryo

dc.contributor.authorSaint-Amant, Louisen_US
dc.contributor.authorDrapeau, Pierreen_US
dc.date.accessioned2006-09-08T21:13:18Z
dc.date.available2006-09-08T21:13:18Z
dc.date.issued2003-03en_US
dc.identifier.citationSaint-Amant, Louis; Drapeau, Pierre; (2003). "Whole-cell patch-clamp recordings from identified spinal neurons in the zebrafish embryo." Methods in Cell Science 25 (1-2): 59-64. <http://hdl.handle.net/2027.42/43239>en_US
dc.identifier.issn1381-5741en_US
dc.identifier.issn1573-0603en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/43239
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=14739588&dopt=citationen_US
dc.description.abstractWe describe a preparation for obtaining patch-clamp recordings from identified embryonic spinal cord interneurons, motoneurons and sensory neurons in an in vivo zebrafish preparation. This preparation is used to study the spatial and temporal patterns of spontaneous and touch-evoked electrical activity during the initial development of circuitry in the spinal cord. The combination of these physiological techniques with the powerful genetic and molecular tools available in the zebrafish has the potential to increase our understanding of the complex interactions between genes and electrical activity during the development of the vertebrate nervous system.en_US
dc.format.extent115443 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherKluwer Academic Publishers; Springer Science+Business Mediaen_US
dc.subject.otherLife Sciencesen_US
dc.subject.otherAnimal Biochemistryen_US
dc.subject.otherPlant Sciencesen_US
dc.subject.otherAnimal Anatomy / Morphology / Histologyen_US
dc.subject.otherEmbryoen_US
dc.subject.otherLocomotionen_US
dc.subject.otherMotor Behavioren_US
dc.subject.otherNeurogenesisen_US
dc.subject.otherPatch-clampen_US
dc.subject.otherSpinal Corden_US
dc.titleWhole-cell patch-clamp recordings from identified spinal neurons in the zebrafish embryoen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Molecular Cellular and Developmental Biology, University of Michigan, 830 North University Ave., Ann Arbor, MI, 48109-1048, USAen_US
dc.contributor.affiliationotherCentre for Research in Neuroscience, McGill University Health Centre Research Institute, Montreal, Quebec, Canada, H3G 1A4en_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid14739588en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/43239/1/11022_2004_Article_5149581.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1023/B:MICS.0000006896.02938.49en_US
dc.identifier.sourceMethods in Cell Scienceen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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