Isolation and sequence of a tomato cDNA clone encoding subunit II of the photosystem I reaction center
dc.contributor.author | Hoffman, Neil E. | en_US |
dc.contributor.author | Malik, Vedpal S. | en_US |
dc.contributor.author | Ko, Kenton | en_US |
dc.contributor.author | Cashmore, Anthony R. | en_US |
dc.contributor.author | Pichersky, Eran | en_US |
dc.date.accessioned | 2006-09-08T21:25:20Z | |
dc.date.available | 2006-09-08T21:25:20Z | |
dc.date.issued | 1988-09 | en_US |
dc.identifier.citation | Hoffman, Neil E.; Pichersky, Eran; Malik, Vedpal S.; Ko, Kenton; Cashmore, Anthony R.; (1988). "Isolation and sequence of a tomato cDNA clone encoding subunit II of the photosystem I reaction center." Plant Molecular Biology 10(5): 435-445. <http://hdl.handle.net/2027.42/43419> | en_US |
dc.identifier.issn | 0167-4412 | en_US |
dc.identifier.issn | 1573-5028 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/43419 | |
dc.description.abstract | We report here the isolation and nucleotide sequence of a cDNA clone encoding a phtosystem I polypeptide that is recognized by a polyclonal antibody prepared against subunit II of the photosystem I reaction center. The transit peptide processing site was determined to occur after Met 50 by N terminal sequencing. The decuced sequence of this protein predicts that the polypeptide has a net positive charge (pI=9.6) and no membrane spanning regions are evident from the hydropathy plot. Based on these considerations and the fact that subunit II is solubilized by alkali treatment of thylakoids, we concluded that subunit II is an extrinsic membrane protein. The absence of hydrophobic regions characteristic of thylakoid transfer domains furthermore implies that subunit II is localized on the stromal side of the membrane. | en_US |
dc.format.extent | 1184955 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Media | en_US |
dc.subject.other | Light-harvesting Complex I | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Plant Sciences | en_US |
dc.subject.other | Plant Pathology | en_US |
dc.subject.other | Electron Transfer | en_US |
dc.subject.other | Membrane Localization | en_US |
dc.subject.other | Photosynthesis | en_US |
dc.subject.other | Processing Site | en_US |
dc.subject.other | Transit Peptide | en_US |
dc.title | Isolation and sequence of a tomato cDNA clone encoding subunit II of the photosystem I reaction center | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biology, University of Michigan, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Plant Science Institute, Department of Biology, University of Pennsylvania, 19104, Philadelphia, PA, USA; Department of Plant Biology, Carnegie Institute of Washington, 290 Panama Street, 94305, Stanford, CA, USA | en_US |
dc.contributor.affiliationother | Plant Science Institute, Department of Biology, University of Pennsylvania, 19104, Philadelphia, PA, USA | en_US |
dc.contributor.affiliationother | Plant Science Institute, Department of Biology, University of Pennsylvania, 19104, Philadelphia, PA, USA | en_US |
dc.contributor.affiliationother | Philip Morris Research Center, P.O. Box 26583, 23261, Richmond, VA, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 24277591 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/43419/1/11103_2004_Article_BF00014949.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00014949 | en_US |
dc.identifier.source | Plant Molecular Biology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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