Rederivation of Transgenic and Gene-Targeted Mice by Embryo Transfer
dc.contributor.author | Van Keuren, Margaret L. | en_US |
dc.contributor.author | Saunders, Thomas L. | en_US |
dc.date.accessioned | 2006-09-11T13:56:21Z | |
dc.date.available | 2006-09-11T13:56:21Z | |
dc.date.issued | 2004-08 | en_US |
dc.identifier.citation | Van Keuren, Margaret L.; Saunders, Thomas L.; (2004). "Rederivation of Transgenic and Gene-Targeted Mice by Embryo Transfer." Transgenic Research 13(4): 363-371. <http://hdl.handle.net/2027.42/43858> | en_US |
dc.identifier.issn | 1573-9368 | en_US |
dc.identifier.issn | 0962-8819 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/43858 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=15517995&dopt=citation | en_US |
dc.description.abstract | Research on genetically engineered mice provides insights into the etiology, therapy, and genetic basis of human diseases. An important variable that affects the results of mouse studies is the health status of the animals. Pathogen burdens may confound observations and obscure underlying mechanisms. Mouse resource centers frequently rederive infected mouse strains. We review our experience on the use of a well-established technique, embryo transfer to rederive infected mouse strains. The following mouse pathogens were eliminated by embryo transfer: Mouse Parvovirus, Mouse Hepatitis Virus, Mouse Rotavirus, Mouse Encephalomyelitis Virus, Mouse Adenovirus, Helicobacter species, endoparasites, and ectoparasites. We rederived transgenic mouse lines, gene-targeted mouse lines, and lines with spontaneous mutations. In the majority of strains, fertilized eggs for embryo transfer were obtained by mating superovulated egg donors with males of the desired genotype. A total of 309 embryo transfers were performed to rederive 96 mouse strains. The pregnancy rate was 76%; 1996 pups were born, of which 43% carried the desired genotype. We performed 44 additional embryo transfers to rederive 15 other strains. The pregnancy rate was lower (45%) and none of the 135 pups carried the desired genotype. Although we successfully eliminated the pathogens in all transfers, we were unable to obtain pups with the desired genotype in 15 of 111 mouse lines. Multiple factors affect the efficiency of rederivation by embryo transfer. They include the response to superovulation by embryo donors, the number and age of stud males, the yield of fertilized eggs, the number of embryo transfers, and genotyping. | en_US |
dc.format.extent | 99831 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Kluwer Academic Publishers ; Springer Science+Business Media | en_US |
dc.subject.other | Animal Anatomy / Morphology / Histology | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Human Genetics | en_US |
dc.subject.other | Plant Sciences | en_US |
dc.subject.other | Embryo Transfer | en_US |
dc.subject.other | Mouse Virus | en_US |
dc.subject.other | Mutation | en_US |
dc.subject.other | Rederivation | en_US |
dc.subject.other | Transgenic Mice | en_US |
dc.subject.other | Pathogen | en_US |
dc.title | Rederivation of Transgenic and Gene-Targeted Mice by Embryo Transfer | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Transgenic Animal Model Core; Department of Internal Medicine, Division of Molecular Medicine and Genetics, University of Michigan Medical School, 2560 MSRB 11 Box 0674, 1150 w.med.center Dr, Ann Arbor, MI, 48109, USA | en_US |
dc.contributor.affiliationother | Transgenic Animal Model Core, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 15517995 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/43858/1/11248_2004_Article_5276068.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/B:TRAG.0000040040.82536.a5 | en_US |
dc.identifier.source | Transgenic Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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