Identification and Cloning of the SNARE Proteins VAMP-2 and Syntaxin-4 from HL-60 Cells and Human Neutrophils
dc.contributor.author | Nauseef, William M. | en_US |
dc.contributor.author | Joe, Youngson | en_US |
dc.contributor.author | Goedken, Melissa | en_US |
dc.contributor.author | Smolen, James E. | en_US |
dc.contributor.author | Hessler, Ronald J. | en_US |
dc.date.accessioned | 2006-09-11T14:56:19Z | |
dc.date.available | 2006-09-11T14:56:19Z | |
dc.date.issued | 2001-08 | en_US |
dc.identifier.citation | Smolen, James E.; Hessler, Ronald J.; Nauseef, William M.; Goedken, Melissa; Joe, Youngson; (2001). "Identification and Cloning of the SNARE Proteins VAMP-2 and Syntaxin-4 from HL-60 Cells and Human Neutrophils." Inflammation 25(4): 255-265. <http://hdl.handle.net/2027.42/44530> | en_US |
dc.identifier.issn | 0360-3997 | en_US |
dc.identifier.issn | 1573-2576 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44530 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=11580102&dopt=citation | en_US |
dc.description.abstract | Degranulation and membrane fusion by neutrophils are essential to host defense. We sought homologues of neuron-specific fusion proteins in human neutrophils and in their precursors, the promyelocytic cell line HL-60. We screened a differentiated HL-60 library and obtained an 848 bp sequence with a 351 bp open reading frame, identical to that published for human VAMP-2 and including 5′ and 3′ untranslated regions. RNA from HL-60 cells during differentiation into the neutrophil lineage was subjected to Northern blot analysis, which revealed a transcript of ∼1050 bp at all stages of differentiation. The amount of these transcripts increased approximately threefold during differentiation, a finding confirmed by quantitative RT-PCR. We also detected mRNA for VAMP-2 in human neutrophils and monocytes using RT-PCR. In like fashion, transcripts of syntaxin-4, another fusion protein, were recovered from a neutrophil cDNA library. As with VAMP-2, expression of syntaxin-4 (determined by Northern blots) also increased, but by only 50%, during differentiation of HL-60 cells. These studies demonstrate that neutrophils and their progenitors possess mRNA for the fusion proteins VAMP-2 and syntaxin-4, and that their transcription increases during differentiation, concurrent with the functional maturation of myeloid cells. | en_US |
dc.format.extent | 158442 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Neutrophil | en_US |
dc.subject.other | Pathology | en_US |
dc.subject.other | Fusion | en_US |
dc.subject.other | Pharmacology/Toxicology | en_US |
dc.subject.other | Medicine & Public Health | en_US |
dc.subject.other | Internal Medicine | en_US |
dc.subject.other | Rheumatology | en_US |
dc.subject.other | VAMP-2 | en_US |
dc.subject.other | Syntaxin-4 | en_US |
dc.subject.other | Synaptobrevin | en_US |
dc.subject.other | HL-60 Cells | en_US |
dc.title | Identification and Cloning of the SNARE Proteins VAMP-2 and Syntaxin-4 from HL-60 Cells and Human Neutrophils | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Pathology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pediatrics, Division of Hematology-Oncology, University of Michigan, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationother | Department of Pediatrics, Leukocyte Biology Section, Baylor College of Medicine, Houston, Texas, 77030-2600; Children's Nutrition Research Center, Baylor College of Medicine, 1100 Bates, Room 6014, Houston, Texas, 77030-2600 | en_US |
dc.contributor.affiliationother | Department of Internal Medicine and Inflammation Program, Veterans Administration Medical Center and University of Iowa, Iowa City, Iowa | en_US |
dc.contributor.affiliationother | Department of Pediatrics, Leukocyte Biology Section, Baylor College of Medicine, Houston, Texas, 77030-2600 | en_US |
dc.contributor.affiliationother | Department of Internal Medicine and Inflammation Program, Veterans Administration Medical Center and University of Iowa, Iowa City, Iowa | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 11580102 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44530/1/10753_2004_Article_342182.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1010903804063 | en_US |
dc.identifier.source | Inflammation | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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