Nonspecific protease and elastase activities in rat leukocytes
dc.contributor.author | Varani, James | en_US |
dc.contributor.author | Ward, D. | en_US |
dc.contributor.author | Johnson, Kent J. | en_US |
dc.date.accessioned | 2006-09-11T14:56:42Z | |
dc.date.available | 2006-09-11T14:56:42Z | |
dc.date.issued | 1982-06 | en_US |
dc.identifier.citation | Varani, J.; Ward, D.; Johnson, K. J.; (1982). "Nonspecific protease and elastase activities in rat leukocytes." Inflammation 6(2): 177-187. <http://hdl.handle.net/2027.42/44534> | en_US |
dc.identifier.issn | 1573-2576 | en_US |
dc.identifier.issn | 0360-3997 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44534 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7049937&dopt=citation | en_US |
dc.description.abstract | Extracts were prepared from rat peritoneal leukocytes obtained 4 h after glycogen injection and assayed for proteolytic enzyme activities against various substrates. The substrates used included acid-denatured bovine hemoglobin, bovine serum albumin, a partially purified preparation of rat pulmonary basement membrane, bovine neck ligament elastin, and an artificial substrate with elastase specificity. A high level of activity was observed when hemoglobin was used as the substrate. The serum albumin and basement membrane preparation were also readily hydrolyzed by the leukocyte extract. In contrast, the native elastin and synthetic elastase substrate were much more resistant. Although the leukocyte extract demonstrated little intrinsic elastase activity, when it was mixed with a commercial hog pancreatic elastase preparation, it greatly potentiated the elastolytic activity, suggesting the activation of a latent enzyme. | en_US |
dc.format.extent | 608853 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Pharmacology/Toxicology | en_US |
dc.subject.other | Pathology | en_US |
dc.subject.other | Medicine & Public Health | en_US |
dc.subject.other | Internal Medicine | en_US |
dc.subject.other | Rheumatology | en_US |
dc.title | Nonspecific protease and elastase activities in rat leukocytes | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Pathology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 7049937 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44534/1/10753_2004_Article_BF00916242.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00916242 | en_US |
dc.identifier.source | Inflammation | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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