Purification and characterization of mouse glucose 6-phosphate dehydrogenase
dc.contributor.author | Yuan, James H. | en_US |
dc.contributor.author | Kramer, James M. | en_US |
dc.contributor.author | Lee, Chi-Yu Gregory | en_US |
dc.contributor.author | Mouer, David | en_US |
dc.date.accessioned | 2006-09-11T15:56:46Z | |
dc.date.available | 2006-09-11T15:56:46Z | |
dc.date.issued | 1979-03 | en_US |
dc.identifier.citation | Lee, Chi-Yu; Yuan, James H.; Mouer, David; Kramer, James M.; (1979). "Purification and characterization of mouse glucose 6-phosphate dehydrogenase." Molecular and Cellular Biochemistry 24(2): 67-73. <http://hdl.handle.net/2027.42/45339> | en_US |
dc.identifier.issn | 1573-4919 | en_US |
dc.identifier.issn | 0300-8177 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/45339 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=460173&dopt=citation | en_US |
dc.description.abstract | Glucose-6-phosphate dehydrogenase was purified to homogeneity from testes and kidneys of the inbred strain of mice (DBA/2J) by a simple two-step affinity column procedure. This involved the sequential application of 8-(6-aminohexyl)-amino-AMP-and -2′, 5′-ADP-Sepharose columns and biospecific elution with NADP + in both steps. The molecular and biochemical properties of the purified enzyme were studied in detail. These include the molecular weight determination, amino acid composition, steady-state kinetics, inactivation by high temperature, urea and iodoacetate, and immunology. The purified enzyme from mouse kidneys or testes was shown to be a tetramer with a molecular weight of 220,000. The enzyme is highly specific for glucose-6-phosphate, exhibits almost no activity with NAD + as a coenzyme and is little inhibited by AMP or ATP. Michaelis constants for glucose-6-phosphate and NADP + were determined to be 50 μm and 10 μm respectively. NADPH is a competitive inhibitor of NADP + and has a K i of 18 µ m . Rabbit antisera against glucose-6-phosphate dehydrogenase were raised. The antisera also cross-react with the same enzyme from human and guinea pig. | en_US |
dc.format.extent | 523294 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Dr. W. Junk b.v. Publishers ; Springer Science+Business Media | en_US |
dc.subject.other | Cardiology | en_US |
dc.subject.other | Oncology | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Medical Biochemistry | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.title | Purification and characterization of mouse glucose 6-phosphate dehydrogenase | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Laboratory of Environmental Mutagenesis, National Institute of Environmental Health Sciences, Research Triangle Park, 27709, North Carolina; Department of Human Genetics, University of Michigan, School of Medicine, 48109, Ann Arber, Michigan | en_US |
dc.contributor.affiliationother | Laboratory of Environmental Mutagenesis, National Institute of Environmental Health Sciences, Research Triangle Park, 27709, North Carolina | en_US |
dc.contributor.affiliationother | Laboratory of Environmental Mutagenesis, National Institute of Environmental Health Sciences, Research Triangle Park, 27709, North Carolina; Department of Chemical Sciences, Old Dominion University, 23508, Norfolk, Virginia | en_US |
dc.contributor.affiliationother | Laboratory of Environmental Mutagenesis, National Institute of Environmental Health Sciences, Research Triangle Park, 27709, North Carolina | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 460173 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/45339/1/11010_2004_Article_BF00314887.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00314887 | en_US |
dc.identifier.source | Molecular and Cellular Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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