Xaf1 can cooperate with TNFα in the induction of apoptosis, independently of interaction with XIAP
dc.contributor.author | Novak, Rachel | en_US |
dc.contributor.author | Duckett, Colin S. | en_US |
dc.contributor.author | Phillips, Andrew C. | en_US |
dc.contributor.author | Xia, Yan | en_US |
dc.contributor.author | Lewis, Jennifer | en_US |
dc.date.accessioned | 2006-09-11T15:56:58Z | |
dc.date.available | 2006-09-11T15:56:58Z | |
dc.date.issued | 2006-06 | en_US |
dc.identifier.citation | Xia, Yan; Novak, Rachel; Lewis, Jennifer; Duckett, Colin S.; Phillips, Andrew C.; (2006). "Xaf1 can cooperate with TNFα in the induction of apoptosis, independently of interaction with XIAP." Molecular and Cellular Biochemistry 286 (1-2): 67-76. <http://hdl.handle.net/2027.42/45342> | en_US |
dc.identifier.issn | 1573-4919 | en_US |
dc.identifier.issn | 0300-8177 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/45342 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16432762&dopt=citation | en_US |
dc.description.abstract | XIAP-associated factor 1 (Xaf1) binds XIAP and re-localizes it to the nucleus, thus inhibiting XIAP activity and enhancing apoptosis [1]. Xaf1 expression is reduced or absent in tumor samples and cell lines suggesting it may function as a tumor suppressor [2–5]. To further study Xaf1 function we generated Xaf1 inducible cells in the osteosarcoma cell line Saos-2. Despite Xaf1 inducing apoptosis that is dramatically enhanced by TNFα we find no evidence for an interaction between Xaf1 and XIAP. Furthermore, Xaf1 expression sensitized XIAP −/− fibroblasts to TNFα, demonstrating the existence of a novel mechanism of Xaf1 induced apoptosis distinct from antagonizing XIAP. Xaf1 expression promotes cytochrome c release that cannot be blocked by inhibition of caspase activity. This implicates a role for the mitochondrial apoptotic pathway, consistent with the ability of Bcl2 to block Xaf1 induced apoptosis. The data indicate that in Saos2 cells Xaf1 activates the mitochondrial apoptotic pathway to facilitate cytochrome c release, thus amplifying apoptotic signals from death receptors. | en_US |
dc.format.extent | 419410 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Springer Science+Business Media, Inc. | en_US |
dc.subject.other | XIAP | en_US |
dc.subject.other | Cytochrome C | en_US |
dc.subject.other | Bcl2 | en_US |
dc.subject.other | Tumor Suppressor | en_US |
dc.subject.other | TNFα | en_US |
dc.subject.other | Caspase | en_US |
dc.subject.other | Xaf1 | en_US |
dc.title | Xaf1 can cooperate with TNFα in the induction of apoptosis, independently of interaction with XIAP | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan, Departments of Pathology and Internal Medicine, University of Michigan Medical School, Room 5315, 1301 Catherine, Ann Arbor, MI, 48109-0602, USA | en_US |
dc.contributor.affiliationum | University of Michigan, Departments of Pathology and Internal Medicine, University of Michigan Medical School, Room 5315, 1301 Catherine, Ann Arbor, MI, 48109-0602, USA | en_US |
dc.contributor.affiliationother | Medical College of Georgia, Institute of Molecular Medicine and Genetics, CB2803, 1120 15th Street, Augusta, GA, 30912, USA | en_US |
dc.contributor.affiliationother | Medical College of Georgia, Institute of Molecular Medicine and Genetics, CB2803, 1120 15th Street, Augusta, GA, 30912, USA | en_US |
dc.contributor.affiliationother | Medical College of Georgia, Institute of Molecular Medicine and Genetics, CB2803, 1120 15th Street, Augusta, GA, 30912, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 16432762 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/45342/1/11010_2005_Article_9094.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s11010-005-9094-2 | en_US |
dc.identifier.source | Molecular and Cellular Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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