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Mutagen-induced diploid human lymphoblast variants containing altered hypoxanthine guanine phosphoribosyl transferase

dc.contributor.authorLittlefield, J. W.en_US
dc.contributor.authorKelley, William N.en_US
dc.contributor.authorEpstein, Joshuaen_US
dc.contributor.authorLeyva, A.en_US
dc.date.accessioned2006-09-11T16:11:39Z
dc.date.available2006-09-11T16:11:39Z
dc.date.issued1977-03en_US
dc.identifier.citationEpstein, J.; Leyva, A.; Kelley, W. N.; Littlefield, J. W.; (1977). "Mutagen-induced diploid human lymphoblast variants containing altered hypoxanthine guanine phosphoribosyl transferase." Somatic Cell Genetics 3(2): 135-148. <http://hdl.handle.net/2027.42/45551>en_US
dc.identifier.issn1572-9931en_US
dc.identifier.issn0098-0366en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/45551
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=605378&dopt=citationen_US
dc.description.abstractThe human lymphoblast line MGL8 was treated with HAT and subsequently “mutagenized” with EMS (200 μg/ml) to give 15% survival, and 6-thioguanine-resistant cells were selected by cloning in soft agarose containing the drug (1 μg/ml). Eighteen sublines of independently derived resistant clones were isolated and studied in detail. One subline had a low residual HGPRT activity of about 1% of the parental cells. The HGPRT of this subline had a higher K m for PRPP, was more sensitive to heat, and was degraded faster by trypsin than the enzyme in extracts of MGL8 cells. This resistant subline and three others contained CRM levels of 1-38%, compared to the wild-type, so they probably represent true structural mutants of the HGPRT gene. All the variants maintained the karyotype of the parental line (46, XY, 6p − ).en_US
dc.format.extent705757 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherKluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Mediaen_US
dc.subject.otherPlant Sciencesen_US
dc.subject.otherHuman Geneticsen_US
dc.subject.otherBiomedicineen_US
dc.subject.otherBiochemistry, Generalen_US
dc.subject.otherAnimal Anatomy / Morphology / Histologyen_US
dc.titleMutagen-induced diploid human lymphoblast variants containing altered hypoxanthine guanine phosphoribosyl transferaseen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Internal Medicine, University of Michigan Medical Center, 48104, Ann Arbor, Michiganen_US
dc.contributor.affiliationumDepartment of Internal Medicine, University of Michigan Medical Center, 48104, Ann Arbor, Michiganen_US
dc.contributor.affiliationotherDepartment of Pediatrics, Johns Hopkins University School of Medicine, 21205, Baltimore, Maryland; Laboratory of Experimental Oncology, Indiana University Medical Centre, 46202, Indianapolis, Indinaen_US
dc.contributor.affiliationotherDepartment of Pediatrics, Johns Hopkins University School of Medicine, 21205, Baltimore, Marylanden_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid605378en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/45551/1/11188_2005_Article_BF01551810.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF01551810en_US
dc.identifier.sourceSomatic Cell Geneticsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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