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| Title: | Chloroplast DNA from lettuce and Barnadesia (Asteraceae): structure, gene localization, and characterization of a large inversion |
| Authors: | Jansen, Robert K.
Palmer, Jeffrey D. |
| Issue Date: | Mar-1987 |
| Publisher: | Springer-Verlag |
| Citation: | Jansen, Robert K; Palmer, Jeffrey D.; (1987). "Chloroplast DNA from lettuce and Barnadesia (Asteraceae): structure, gene localization, and characterization of a large inversion." Current Genetics 11 (6-7): 553-564. <http://hdl.handle.net/2027.42/46961> |
| Abstract: | We have cloned into plasmids 17 of 18 lettuce chloroplast DNA SacI fragments covering 96% of the genome. The cloned fragments were used to construct cleavage maps for 10 restriction enzymes for the chloroplast genomes of lettuce ( Lactuca sativa ) and Barnadesia caryophylla , two distantly related species in the sunflower family (Asteraceae). Both genomes are approximately 151 kb in size and contain a 25 kb inverted repeat. We also mapped the position and orientation of 37 chloroplast DNA genes. The mapping studies reveal that chloroplast DNAs of lettuce and Barnadesia differ by a 22 kb inversion in the large single copy region. Barnadesia has retained the primitive land plant genome arrangement, while the inversion has occurred in a lettuce lineage. The endpoints of the derived lettuce inversion were located by comparison to the well-characterized spinach and tobacco genomes. Both endpoints are located in intergenic spacers within tRNA gene clusters; one cluster being located downstream from the atpA gene and the other upstream from the psbD gene. The endpoint near the atpA gene is very close to one endpoint of a 20 kb inversion in wheat (Howe et al. 1983; Quigley and Weil 1985). Comparison of the restriction site maps gives an estimated sequence divergence of 3.7% for the lettuce and Barnadesia genomes. This value is relatively low compared to previous estimates for other angiosperm groups, suggesting a high degree of sequence conservation in the Asteraceae. |
| ISSN: | 0172-8083
1432-0983 |
| DOI: | 10.1007/BF00384619 |
| Appears in Collections: | Interdisciplinary and Peer-Reviewed
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| 294_2004_Article_BF00384619.pdf | | 939Kb | Adobe PDF | View/Open |
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