Protease inhibitors of Manduca sexta expressed in transgenic cotton
dc.contributor.author | Thomas, John C. | en_US |
dc.contributor.author | Brown, Judith K. | en_US |
dc.contributor.author | Adams, Deanna G. | en_US |
dc.contributor.author | Keppenne, Veronique D. | en_US |
dc.contributor.author | Wasmann, Catherine C. | en_US |
dc.contributor.author | Kanost, Michael R. | en_US |
dc.contributor.author | Bohnert, Hans J. | en_US |
dc.date.accessioned | 2006-09-11T18:27:05Z | |
dc.date.available | 2006-09-11T18:27:05Z | |
dc.date.issued | 1995-10 | en_US |
dc.identifier.citation | Thomas, John C.; Adams, Deanna G.; Keppenne, Veronique D.; Wasmann, Catherine C.; Brown, Judith K.; Kanost, Michael R.; Bohnert, Hans J.; (1995). "Protease inhibitors of Manduca sexta expressed in transgenic cotton." Plant Cell Reports 14(12): 758-762. <http://hdl.handle.net/2027.42/46980> | en_US |
dc.identifier.issn | 0721-7714 | en_US |
dc.identifier.issn | 1432-203X | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/46980 | |
dc.description.abstract | To explore the effectiveness of insect derived protease inhibitors in protecting plants against insect feeding, anti-trypsin, anti-chymotrypsin and anti-elastase protease inhibitor (PI) genes from Manduca sexta L. were expressed in transgenic cotton ( Gossypium hirsutum L.). From 198 independent transformants, 35 elite lines were further analyzed. Under the control of the 35S promoter of CaMV, PI accumulated to approximately 0.1% of total protein, depending on the tissue analyzed. Using cell-flow cytometry, DNA content/ nuclei of transgenic and non-transformed cotton were identical. On cotton plants expressing PIs, fecundity of Bemisia tabaci (Genn.), the sweetpotato whitefly, was reduced compared to controls. Expression of these protease inhibitors may reduce the developmental rate of B. tabaci and other insects, and provide a strategy for cotton protection. | en_US |
dc.format.extent | 703456 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Cell Biology | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Plant Transformation | en_US |
dc.subject.other | Biotechnology | en_US |
dc.subject.other | Protease Inhibitor | en_US |
dc.title | Protease inhibitors of Manduca sexta expressed in transgenic cotton | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biochemistry, University of Arizona, 85721, Tucson, AZ, USA; Department of Natural Sciences, University of Michigan-Dearborn, 48128-1491, Dearborn, MI, USA | en_US |
dc.contributor.affiliationother | Department of Plant Pathology, University of Arizona, 85721, Tucson, AZ, USA | en_US |
dc.contributor.affiliationother | Department of Plant Sciences, University of Arizona, 85721, Tucson, AZ, USA | en_US |
dc.contributor.affiliationother | Department of Biochemistry, University of Arizona, 85721, Tucson, AZ, USA; Department of Plant Sciences, University of Arizona, 85721, Tucson, AZ, USA | en_US |
dc.contributor.affiliationother | Department of Biochemistry, University of Arizona, 85721, Tucson, AZ, USA | en_US |
dc.contributor.affiliationother | Department of Biochemistry, Kansas State University, 66506-0001, Manhattan, KS, USA | en_US |
dc.contributor.affiliationother | Department of Plant Sciences, University of Arizona, 85721, Tucson, AZ, USA | en_US |
dc.contributor.affiliationumcampus | Dearborn | en_US |
dc.identifier.pmid | 24186707 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/46980/1/299_2004_Article_BF00232917.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00232917 | en_US |
dc.identifier.source | Plant Cell Reports | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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