Phosphorylation-dependent neurofilament epitopes are reduced at the node of Ranvier
dc.contributor.author | Mata, Marina | en_US |
dc.contributor.author | Kupina, Nancy C. | en_US |
dc.contributor.author | Fink, David J. | en_US |
dc.date.accessioned | 2006-09-11T18:57:55Z | |
dc.date.available | 2006-09-11T18:57:55Z | |
dc.date.issued | 1992-03 | en_US |
dc.identifier.citation | Mata, M.; Kupina, N.; Fink, D. J.; (1992). "Phosphorylation-dependent neurofilament epitopes are reduced at the node of Ranvier." Journal of Neurocytology 21(3): 199-210. <http://hdl.handle.net/2027.42/47426> | en_US |
dc.identifier.issn | 0300-4864 | en_US |
dc.identifier.issn | 1573-7381 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/47426 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1373184&dopt=citation | en_US |
dc.description.abstract | Neurofilaments in axons are highly phosphorylated at multiple sites on the 200 kDa neurofilament (neurofilament-H) and 160 kDa (neurofilament-M) subunit peptides. We used a panel of monoclonal and polyclonal antibodies against distinct neurofilament epitopes to study the distribution of these epitopes along the axons of large myelinated fibres in rat sciatic nerve using quantitative electron microscopic immunocytochemistry with colloidal gold. Antibodies specific for phosphorylated epitopes on neurofilament-H showed a 60% reduction in density of immunoreactivity at the node of Ranvier, compared to the internodal axon. Antibodies directed against neurofilament-M, which recognized phosphorylated epitopes preferentially, showed a 40% reduction in density of immunoreactivity at the node. Following dephosphorylation of the neurofilaments in tissue sections by alkaline phosphatase treatment, antibodies which recognized dephosphorylated forms of neurofilament-H showed no reduction in density of immunoreactivity at the node. Similarly, an antibody directed against the 70 kDa subunit (neurofilament-L), showed no reduction in density of immunoreactivity at the node. | en_US |
dc.format.extent | 5989920 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Chapman and Hall Ltd ; Springer Science+Business Media | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Neurosciences | en_US |
dc.subject.other | Neuroradiology | en_US |
dc.title | Phosphorylation-dependent neurofilament epitopes are reduced at the node of Ranvier | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan and GRECC Neurology Research Laboratory, VAMC, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan and GRECC Neurology Research Laboratory, VAMC, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan and GRECC Neurology Research Laboratory, VAMC, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 1373184 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/47426/1/11068_2005_Article_BF01194978.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF01194978 | en_US |
dc.identifier.source | Journal of Neurocytology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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