A point mutation in the Nul gene of bacteriophage λ facilitates phage growth in Escherichia coli with himA and gyrB mutations
dc.contributor.author | Friedman, David I. | en_US |
dc.contributor.author | Eades, Linda J. | en_US |
dc.contributor.author | Alessi, Diane M. | en_US |
dc.contributor.author | Granston, Andrew E. | en_US |
dc.date.accessioned | 2006-09-11T19:07:53Z | |
dc.date.available | 2006-09-11T19:07:53Z | |
dc.date.issued | 1988-04 | en_US |
dc.identifier.citation | Granston, Andrew E.; Alessi, Diane M.; Eades, Linda J.; Friedman, David I.; (1988). "A point mutation in the Nul gene of bacteriophage λ facilitates phage growth in Escherichia coli with himA and gyrB mutations." MGG Molecular & General Genetics 212(1): 149-156. <http://hdl.handle.net/2027.42/47567> | en_US |
dc.identifier.issn | 0026-8925 | en_US |
dc.identifier.issn | 1617-4623 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/47567 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2836702&dopt=citation | en_US |
dc.description.abstract | A mutant of λ was isolated that grows in the Escherichia coli himAΔ/gyrB-him320 (Ts) double mutant at 42°C; conditions which are non-permissive for wild-type λ growth. The responsible mutation, ohm1 , alters the 40th codon of the Nul reading frame. The Nul and A gene products comprise the terminase protein which cleaves concatameric DNA into unit-length phage genomes during DNA packaging. The Nul-ohm1 gene product acts in trans to support λ growth in the double himA/gyrB mutant, and λ cos154 growth in the single himA mutant. The observation that an alteration in Nul suppresses the inhibition of growth in the double himA/gyrB mutant implicates DNA gyrase, as well as integration host factor, in the DNA: protein interactions that occur at the initiation of packaging. | en_US |
dc.format.extent | 848461 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | DNA Packaging | en_US |
dc.subject.other | Cell Biology | en_US |
dc.subject.other | DNA Gyrase | en_US |
dc.subject.other | Terminase | en_US |
dc.subject.other | Integration Host Factor | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Microbial Genetics and Genomics | en_US |
dc.subject.other | Cos | en_US |
dc.title | A point mutation in the Nul gene of bacteriophage λ facilitates phage growth in Escherichia coli with himA and gyrB mutations | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 2836702 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/47567/1/438_2004_Article_BF00322458.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00322458 | en_US |
dc.identifier.source | MGG Molecular & General Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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