An improved method for detecting Y chromosomal DNA
dc.contributor.author | Stalvey, John R. D. | en_US |
dc.contributor.author | Erickson, Robert P. | en_US |
dc.date.accessioned | 2006-09-11T19:11:45Z | |
dc.date.available | 2006-09-11T19:11:45Z | |
dc.date.issued | 1987-07 | en_US |
dc.identifier.citation | Stalvey, John R. D.; Erickson, Robert P.; (1987). "An improved method for detecting Y chromosomal DNA." Human Genetics 76(3): 240-243. <http://hdl.handle.net/2027.42/47617> | en_US |
dc.identifier.issn | 1432-1203 | en_US |
dc.identifier.issn | 0340-6717 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/47617 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3036688&dopt=citation | en_US |
dc.description.abstract | The DNA probe Y97 was derived from a repeat sequence in the human Y centromere, a region which must be present in a mitotically functional Y chromosome. We have demonstrated that Y97, which detects a Y-specific 5.5-kb Eco RI fragment by Southern analysis, is very useful for the molecular detection of small amounts of Y-derived material and represents a significant improvement over previous tests for molecular diagnosis of sex. The male-female difference in hybridization was unequivocal even when only 25 ng of total DNA was used per lane. Furthermore, in mixing experiments the 5.5-kb Eco RI fragment was detectable even when only 5% of the total DNA was male. By increasing hybridization stringency, we have developed a rapid, sensitive, and accurate method to detect Y chromosomal DNA in unrestricted samples. | en_US |
dc.format.extent | 655119 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Molecular Medicine | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Human Genetics | en_US |
dc.subject.other | Internal Medicine | en_US |
dc.subject.other | Metabolic Diseases | en_US |
dc.title | An improved method for detecting Y chromosomal DNA | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Pediatric Genetics, Department of Pediatrics and Communicable Diseases, University of Michigan School of Medicine, 48109-0618, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Division of Pediatric Genetics, Department of Pediatrics and Communicable Diseases, University of Michigan School of Medicine, 48109-0618, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 3036688 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/47617/1/439_2004_Article_BF00283615.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00283615 | en_US |
dc.identifier.source | Human Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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