|
Deep Blue at the University of Michigan >
Research Collections >
Interdisciplinary and Peer-Reviewed >
|
Please use this persistent URL to cite or link to this item:
|
| Title: | Localization of the transcriptional coactivator PGC-1Α to GABAergic neurons during maturation of the rat brain |
| Authors: | Cowell, Rita Marie
Blake, Kathryn Rose
Russell, James W. |
| Issue Date: | 1-May-2007 |
| Publisher: | Wiley Subscription Services, Inc., A Wiley Company |
| Citation: | Cowell, Rita Marie; Blake, Kathryn Rose; Russell, James W. (2007). "Localization of the transcriptional coactivator PGC-1Α to GABAergic neurons during maturation of the rat brain." The Journal of Comparative Neurology 502(1): 1-18. <http://hdl.handle.net/2027.42/55932> |
| Abstract: | The transcriptional coactivator peroxisome proliferator activated receptor Γ coactivator 1Α (PGC-1Α) can activate a number of transcription factors to regulate mitochondrial biogenesis and cell-specific responses to cold, fasting, and exercise. Recent studies indicate that PGC-1Α knockout mice exhibit behavioral abnormalities and progressive vacuolization in various brain regions. To investigate the roles for PGC-1Α in the nervous system, we evaluated the temporal and cell-specific expression of PGC-1Α in the normal developing rat brain. Western blot of whole brain homogenates with a PGC-1Α-specific antibody revealed that PGC-1Α protein was most abundant in the embryonic and early postnatal forebrain and cerebellum. Using quantitative reverse-transcriptase polymerase chain reaction (RT-PCR), we determined that PGC-1Α mRNA expression increased most markedly between postnatal days 3 (P3) and 14 in the cortex, striatum, and hippocampus. Immunohistochemical and immunofluorescence analyses of brain tissue indicated that while PGC-1Α was found in most neuronal populations from embryonic day 15 to P3, it was specifically concentrated in GABAergic populations from P3 to adulthood. Interestingly, PGC-1Α colocalized with the developmentally regulated chemoattractant reelin in the cortex and hippocampus, and the survival-promoting transcription factor myocyte enhancing factor 2 was highly concentrated in GABAergic populations in the striatum and cerebellum at times of PGC-1Α expression. These results implicate PGC-1Α as a regulator of metabolism and/or survival in GABAergic neurons during a phase of mitochondrial and synaptic changes in the developing brain and suggest that PGC-1Α may be a good target for increasing metabolism in GABAergic populations in neurodevelopmental and neurodegenerative disorders. J. Comp. Neurol. 502:1–18, 2007. © 2007 Wiley-Liss, Inc. |
| URI: | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db
=pubmed&list_uids=17335037&dopt=citation |
| ISSN: | 0021-9967
1096-9861 |
| DOI: | 10.1002/cne.21211 |
| PMID: | 17335037 |
| Appears in Collections: | Interdisciplinary and Peer-Reviewed
|
Files in This Item:
| File |
Description |
Size | Format | |
|---|
| 21211_ftp.pdf | | 4333Kb | Adobe PDF | View/Open |
|
Deep Blue encourages the fair use of copyrighted material, and you are free to link to content here without asking for permission. Consult the document(s) and/or contact the copyright holder for additional rights questions and requests.
|
