Raman spectral signatures of mouse mammary tissue and associated lymph nodes: normal, tumor and mastitis
dc.contributor.author | Thakur, Jagdish S. | en_US |
dc.contributor.author | Dai, Houbei | en_US |
dc.contributor.author | Serhatkulu, Gulay K. | en_US |
dc.contributor.author | Naik, Ratna | en_US |
dc.contributor.author | Naik, Vaman M. | en_US |
dc.contributor.author | Cao, Alex | en_US |
dc.contributor.author | Pandya, Abhilash K. | en_US |
dc.contributor.author | Auner, Gregory W. | en_US |
dc.contributor.author | Rabah, Rajah | en_US |
dc.contributor.author | Klein, Michael D. | en_US |
dc.contributor.author | Freeman, Carl | en_US |
dc.date.accessioned | 2007-09-20T18:12:53Z | |
dc.date.available | 2008-04-03T18:49:56Z | en_US |
dc.date.issued | 2007-02 | en_US |
dc.identifier.citation | Thakur, Jagdish S.; Dai, Houbei; Serhatkulu, Gulay K.; Naik, Ratna; Naik, Vaman M.; Cao, Alex; Pandya, Abhilash; Auner, Gregory W.; Rabah, Rajah; Klein, Michael D.; Freeman, Carl (2007). "Raman spectral signatures of mouse mammary tissue and associated lymph nodes: normal, tumor and mastitis." Journal of Raman Spectroscopy 38(2): 127-134. <http://hdl.handle.net/2027.42/55947> | en_US |
dc.identifier.issn | 0377-0486 | en_US |
dc.identifier.issn | 1097-4555 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/55947 | |
dc.description.abstract | Raman spectroscopy involves the interaction of light with the molecular vibrations and therefore can provide information about molecular structure, tissue composition and changes in its environment. We explored whether Raman spectroscopy can reliably distinguish mammary tumors from normal mammary tissues and other pathological states in mice. We analyzed a large number of Raman spectra from the tumor and normal mammary glands of mice injected with 4T1 tumor cells, which were collected using a high-resolution (less than 4 cm −1 ) Raman spectrometer at a fixed (785 nm) laser excitation wavelength and with 60 mW of laser power. The spectra of normal and tumor mammary glands showed consistent differences in the intensity of certain Raman bands and loss of some bands in the tumor spectra. Multivariate statistical methods—principal component analysis (PCA) and discriminant functional analysis (DFA)—were used to separate the data into different groups of mammary tumors, mastitis, lymph nodes contralateral and tumor-cell-injected sides, and normal contralateral and tumor-cell-injected sides. We demonstrate that this spectroscopic technique has the feasibility of discriminating tumor and mastitis from normal tissues and other pathological states in a short period of time and may detect tumor transformation earlier than the standard histological examination stage. Copyright © 2006 John Wiley & Sons, Ltd. | en_US |
dc.format.extent | 1006758 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | John Wiley & Sons, Ltd. | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Analytical Chemistry and Spectroscopy | en_US |
dc.title | Raman spectral signatures of mouse mammary tissue and associated lymph nodes: normal, tumor and mastitis | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Physics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Natural Sciences, University of Michigan-Dearborn, Dearborn, MI 48128, USA | en_US |
dc.contributor.affiliationother | Department of Electrical and Computer Engineering, Wayne State University, Detroit, MI 48202, USA ; Department of Electrical and Computer Engineering, Wayne State University, Detroit, MI 48202, USA. | en_US |
dc.contributor.affiliationother | Department of Physics and Astronomy, Wayne State University, Detroit, MI 48202, USA | en_US |
dc.contributor.affiliationother | Department of Electrical and Computer Engineering, Wayne State University, Detroit, MI 48202, USA | en_US |
dc.contributor.affiliationother | Department of Physics and Astronomy, Wayne State University, Detroit, MI 48202, USA | en_US |
dc.contributor.affiliationother | Department of Electrical and Computer Engineering, Wayne State University, Detroit, MI 48202, USA | en_US |
dc.contributor.affiliationother | Department of Electrical and Computer Engineering, Wayne State University, Detroit, MI 48202, USA | en_US |
dc.contributor.affiliationother | Department of Electrical and Computer Engineering, Wayne State University, Detroit, MI 48202, USA | en_US |
dc.contributor.affiliationother | Departments of Surgery (Pediatric Surgery) and Pathology, Wayne State University School of Medicine and the Children's Hospital of Michigan, Detroit, MI 48201, USA | en_US |
dc.contributor.affiliationother | Departments of Surgery (Pediatric Surgery) and Pathology, Wayne State University School of Medicine and the Children's Hospital of Michigan, Detroit, MI 48201, USA | en_US |
dc.contributor.affiliationother | Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/55947/1/1565_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/jrs.1565 | en_US |
dc.identifier.source | Journal of Raman Spectroscopy | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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