An experimental strategy for quantitative analysis of the humoral immune response to prostate cancer antigens using natural protein microarrays
dc.contributor.author | Forrester, Sara | en_US |
dc.contributor.author | Qiu, Ji | en_US |
dc.contributor.author | Mangold, Leslie | en_US |
dc.contributor.author | Partin, Alan W. | en_US |
dc.contributor.author | Misek, David E. | en_US |
dc.contributor.author | Phinney, Brett | en_US |
dc.contributor.author | Whitten, Douglas | en_US |
dc.contributor.author | Andrews, Philip | en_US |
dc.contributor.author | Diamandis, Eleftherios | en_US |
dc.contributor.author | Omenn, Gilbert S. | en_US |
dc.contributor.author | Hanash, Samir M. | en_US |
dc.contributor.author | Haab, Brian B. | en_US |
dc.date.accessioned | 2007-09-20T18:55:48Z | |
dc.date.available | 2008-09-08T14:25:12Z | en_US |
dc.date.issued | 2007-05 | en_US |
dc.identifier.citation | Forrester, Sara; Qiu, Ji; Mangold, Leslie; Partin, Alan; Misek, David; Phinney, Brett; Whitten, Douglas; Andrews, Philip; Diamandis, Eleftherios; Omenn, Gilbert S.; Hanash, Samir; Haab, Brian B. (2007)."An experimental strategy for quantitative analysis of the humoral immune response to prostate cancer antigens using natural protein microarrays." PROTEOMICS - Clinical Applications 1(5): 494-505. <http://hdl.handle.net/2027.42/56104> | en_US |
dc.identifier.issn | 1862-8346 | en_US |
dc.identifier.issn | 1862-8354 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/56104 | |
dc.description.abstract | The identification of human tumor antigens has potential utility in the diagnosis and treatment of cancers. We demonstrate here a complete strategy to profile immunoreactivity and identify tumor antigens from proteins derived from tumor cell lines. Microarrays of proteins produced from 2-D LC fractionation of prostate tumor cell-line lysates were used to profile immunoreactivity in the sera of prostate cancer patients and control subjects. Cancer-associated immunoreactivity to distinct groups of chromatography fractions was present in about 50% of the patients, with greater immunoreactivity present in patients with non-organ-confined cancer than in patients with organ-confined cancer. We grouped the immunoreactive fractions by similarities in elution order and patterns of immunoreactivity to guide and interpret the MS analysis of selected fractions, which was used to identify the proteins that may be responsible for the immunoreactivity. As a complementary method to further characterize and validate the immunoreactivity of the proteins identified by mass spectrometry, we demonstrate the use of focused microarrays of recombinant proteins. Disease-associated immunoreactivity was confirmed for one of the identified proteins, human Kallikrein 11. These results demonstrate a practical approach to screening, identifying, and validating immunoreactive proteins that could be applied to diverse studies on humoral immune responses. | en_US |
dc.format.extent | 776579 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | WILEY-VCH Verlag | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Molecular Cell Biology | en_US |
dc.title | An experimental strategy for quantitative analysis of the humoral immune response to prostate cancer antigens using natural protein microarrays | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Medicine (General) | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan Medical School, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | University of Michigan Medical School, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | University of Michigan Medical School, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Van Andel Research Institute, Grand Rapids, MI, USA | en_US |
dc.contributor.affiliationother | Fred Hutchinson Cancer Research Center, Seattle, WA, USA | en_US |
dc.contributor.affiliationother | John Hopkins University School of Medicine, Baltimore, MD, USA | en_US |
dc.contributor.affiliationother | John Hopkins University School of Medicine, Baltimore, MD, USA | en_US |
dc.contributor.affiliationother | Michigan State University, East Lansing, MI, USA ; Present address: University of California Davis Genome Center, Genome Biomedical Sciences Facility, Davis, CA, USA | en_US |
dc.contributor.affiliationother | Michigan State University, East Lansing, MI, USA | en_US |
dc.contributor.affiliationother | University of Toronto, Toronto, Ontario, Canada | en_US |
dc.contributor.affiliationother | Fred Hutchinson Cancer Research Center, Seattle, WA, USA | en_US |
dc.contributor.affiliationother | Van Andel Research Institute, Grand Rapids, MI, USA ; Van Andel Research Institute, 333 Bostwick, Grand Rapids, MI 49503, USA Fax : +1-616-234-5269 | en_US |
dc.identifier.pmid | 21136701 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/56104/1/494_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/prca.200600802 | en_US |
dc.identifier.source | PROTEOMICS - Clinical Applications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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