Critical role for Ipaf in Pseudomonas aeruginosa -induced caspase-1 activation
dc.contributor.author | Franchi, Luigi | en_US |
dc.contributor.author | Stoolman, Joshua S. | en_US |
dc.contributor.author | Kanneganti, Thirumala-Devi | en_US |
dc.contributor.author | Verma, Amrisha | en_US |
dc.contributor.author | Ramphal, Reuben | en_US |
dc.date.accessioned | 2007-12-04T18:25:42Z | |
dc.date.available | 2008-11-05T15:05:43Z | en_US |
dc.date.issued | 2007-11 | en_US |
dc.identifier.citation | Franchi, Luigi; Stoolman, Joshua; Kanneganti, Thirumala-Devi; Verma, Amrisha; Ramphal, Reuben (2007). "Critical role for Ipaf in Pseudomonas aeruginosa -induced caspase-1 activation." European Journal of Immunology 37(11): 3030-3039. <http://hdl.handle.net/2027.42/57332> | en_US |
dc.identifier.issn | 0014-2980 | en_US |
dc.identifier.issn | 1521-4141 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/57332 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=17935074&dopt=citation | |
dc.description.abstract | Pseudomonas aeruginosa is an opportunistic Gram-negative human pathogen that is responsible for a broad range of infections in individuals with a variety of predisposing conditions. After infection, P. aeruginosa induces a marked inflammatory response in the host. However the mechanisms involved in bacterium recognition and induction of immune responses are poorly understood. Here we report that the Nod-like receptor family member Ipaf is required for optimal bacterial clearance in an in vivo model of P. aeruginosa lung infection. Further analysis showed that bacterial flagellin was essential for caspase-1 and IL-1β and this activity depended on Ipaf and the adaptor ASC but not TLR5. Notably, P. aeruginosa induced macrophage cell death and this event relied on flagellin and Ipaf but not on ASC. Analysis of Pseudomonas mutants revealed that different amino acid residues of flagellin were critical for sensing by Ipaf and TLR5. Finally, activation of caspase-1 and IL-1β secretion by P. aeruginosa required a functional type III secretion system, but not the effector molecules ExoS, ExoT and ExoY. These results provide new insight into the interaction of P. aeruginosa with host macrophages and suggest that distinct regions of flagellin are sensed by Ipaf and TLR5. | en_US |
dc.format.extent | 367974 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | WILEY-VCH Verlag | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Microbiology and Immunology | en_US |
dc.title | Critical role for Ipaf in Pseudomonas aeruginosa -induced caspase-1 activation | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology and Comprehensive Cancer Center, The University of Michigan Medical School, Ann Arbor, USA | en_US |
dc.contributor.affiliationum | Department of Pathology and Comprehensive Cancer Center, The University of Michigan Medical School, Ann Arbor, USA | en_US |
dc.contributor.affiliationum | Department of Pathology and Comprehensive Cancer Center, The University of Michigan Medical School, Ann Arbor, USA | en_US |
dc.contributor.affiliationother | Department of Medicine, University of Florida, Gainesville, USA | en_US |
dc.contributor.affiliationother | Department of Medicine, University of Florida, Gainesville, USA | en_US |
dc.identifier.pmid | 17935074 | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/57332/1/3030_ftp.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1002/eji.200737532 | en_US |
dc.identifier.source | European Journal of Immunology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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